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Excitement Over Automated Patch Clamp: Action Potentials from Cardiac Myocytes

Authors :
Claudia Haarmann
Niels Fertig
Andrea Brüggemann
Alison Haythornthwaite
Michael George
Ralf Kettenhofen
Sonja Stoelzle
Cecilia Farre
David Guinot
Source :
Biophysical Journal. (3):514a
Publisher :
Biophysical Society. Published by Elsevier Inc.

Abstract

The use of cardiac myocytes is becoming increasingly important for drug safety testing. Unique features of certain planar patch clamp workstations, coupled with ease-of-use and higher data throughput, make these devices ideal tools for ion channel screening and safety testing. Using stem cell derived cardiac myocytes, recordings could be made not only in the voltage-clamp mode but also in the current-clamp mode on a planar patch clamp workstation. This demonstrates for the first time parallel current-clamp recordings on a planar patch clamp workstation. Ion channels important in drug discovery, such as hERG and voltage-gated Na+, Ca2+ and K+ channels in the voltage-clamp mode from stem cell derived cardiac myocytes will be shown. In addition, action potential recordings in the current-clamp mode at 35°C, and modulation of the action potentials by hERG active compounds, will also be shown.View Large Image | View Hi-Res Image | Download PowerPoint Slide

Details

Language :
English
ISSN :
00063495
Issue :
3
Database :
OpenAIRE
Journal :
Biophysical Journal
Accession number :
edsair.doi.dedup.....68868fc8b48e91f4a10d0b0d6bdc4346
Full Text :
https://doi.org/10.1016/j.bpj.2009.12.2798