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Abnormalities in the p34cdc2-Related PITSLRE Protein Kinase Gene Complex (CDC2L) on Chromosome Band 1p36 in Melanoma
- Source :
- Cancer Genetics and Cytogenetics. 108:91-99
- Publication Year :
- 1999
- Publisher :
- Elsevier BV, 1999.
-
Abstract
- The two genes encoding the PITSLRE protein kinase isoforms, CDC2L1 and CDC2L2, are localized to human chromosome band 1p36. The PITSLRE protein kinases are a part of the p34cdc2 supergene family. Several protein products of the CDC2L locus may be effector(s) in apoptotic signaling. The larger PITSLRE p110 isoforms appear to regulate some aspect of RNA splicing/transcription during the cell cycle. One or more of these genes may function as tumor suppressor genes in melanoma. Using fluorescence in situ hybridization, one allele of the CDC2L gene complex on chromosome 1 was either deleted or translocated in 8 of 14 different melanoma cell lines. We also observed mutations in the 5' promoter region of the CDC2L1 gene in four different cell lines relative to normal melanocytes using PCR-SSCP analysis and direct DNA sequencing. Western blot analysis revealed decreased level of PITSLRE protein expression in several cell lines, as well as in four surgical malignant melanoma specimens relative to normal melanocytes. Thus, the decreased PITSLRE protein expression appears to result from deletion of the CDC2L alleles and possibly by mutations within the 5' promoter region. We propose that aberrations in the CDC2L genes may contribute to the pathogenesis or progression of melanoma.
- Subjects :
- Cancer Research
Tumor suppressor gene
Locus (genetics)
Protein Serine-Threonine Kinases
Biology
medicine.disease_cause
Polymerase Chain Reaction
Gene expression
Tumor Cells, Cultured
Genetics
medicine
Humans
Protein kinase A
Melanoma
Molecular Biology
Gene
In Situ Hybridization
Polymorphism, Single-Stranded Conformational
Base Sequence
Promoter
DNA, Neoplasm
Cell cycle
Molecular biology
Cyclin-Dependent Kinases
Chromosomes, Human, Pair 1
Mutation
Carcinogenesis
Protein Kinases
Subjects
Details
- ISSN :
- 01654608
- Volume :
- 108
- Database :
- OpenAIRE
- Journal :
- Cancer Genetics and Cytogenetics
- Accession number :
- edsair.doi.dedup.....688437003e094e036b062e584e6a1333