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A SERI technique reveals an immunosuppressive activity of a serine-rich protein encoded in Cotesia plutellae bracovirus
- Source :
- BMB Reports. 43:279-283
- Publication Year :
- 2010
- Publisher :
- Korean Society for Biochemistry and Molecular Biology - BMB Reports, 2010.
-
Abstract
- Polydnavirus genome is segmented and dispersed on host wasp chromosome. After replication, the segments form double-stranded circular DNAs and embedded in viral coat proteins. These viral particles are delivered into a parasitized host along with parasitoid eggs. A serine-rich protein (SRP) is predicted in a polydnavirus, Cotesia plutellae bracovirus (CpBV), genome in its segment no. 33 (CpBV-S33), creating CpBV- SRP1. This study explored its expression and physiological function in the diamondback moth, Plutella xylostella, larvae parasitized by C. plutellae. CpBV-SRP1 encodes 122 amino acids with 26 serines and several predicted phosphorylation sites. It is persistently expressed in all tested tissues of parasitized P. xylostella including hemocyte, fat body, and gut. Its physiological function was analyzed by injecting CpBV-S33 and inducing its expression in nonparasitized P. xylostella by a technique called SERI (segment expression and RNA interference). The expression of CpBV-SRP1 significantly impaired the spreading behavior and total cell count of hemocytes of treated larvae. Subsequent RNA interference of CpBV-SRP1 rescued the immunosuppressive response. This study reports the persistent expression of CpBV-SRP1 in a parasitized host and its parasitic role in suppressing the host immune response by altering hemocyte behavior and survival. [BMB reports 2010; 43(4): 279-283]
- Subjects :
- Hemocytes
Molecular Sequence Data
Wasps
Moths
Biochemistry
Genome
Serine
Viral Proteins
chemistry.chemical_compound
Immune system
RNA interference
Immune Tolerance
Animals
Amino Acid Sequence
Phosphorylation
Molecular Biology
Diamondback moth
Sequence Homology, Amino Acid
biology
Host (biology)
Polydnavirus
General Medicine
biology.organism_classification
Virology
Cell biology
chemistry
Polydnaviridae
Larva
RNA Interference
Sequence Alignment
DNA
Subjects
Details
- ISSN :
- 19766696
- Volume :
- 43
- Database :
- OpenAIRE
- Journal :
- BMB Reports
- Accession number :
- edsair.doi.dedup.....687ca660319b1a58255b4d59d31aef18