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EXCITATION OF MURINE CARDIAC MYOCYTES BY NANOSECOND PULSED ELECTRIC FIELD
- Publication Year :
- 2019
-
Abstract
- INTRODUCTION Opening of voltage-gated sodium channels takes tens to hundreds of microseconds, and mechanisms of their opening by nanosecond pulsed electric field (nsPEF) stimuli remain elusive. This study was aimed at uncovering the mechanisms of how nsPEF elicits action potentials (APs) in cardiomyocytes. METHODS AND RESULTS Fluorescent imaging of optical APs (FluoVolt) and Ca2+ -transients (Fluo-4) was performed in enzymatically isolated murine ventricular cardiomyocytes stimulated by 200-nanosecond trapezoidal pulses. nsPEF stimulation evoked tetrodotoxin-sensitive APs accompanied or preceded by slow sustained depolarization (SSD) and, in most cells, by transient afterdepolarization waves. SSD threshold was lower than the AP threshold (1.26 ± 0.03 vs 1.34 ± 0.03 kV/cm, respectively, P
- Subjects :
- Time Factors
Calcium Channels, L-Type
chemistry.chemical_element
Action Potentials
030204 cardiovascular system & hematology
Calcium
Article
Sodium-Calcium Exchanger
Afterdepolarization
03 medical and health sciences
0302 clinical medicine
Physiology (medical)
medicine
Myocyte
Animals
Myocytes, Cardiac
030212 general & internal medicine
Calcium Signaling
Ion channel
Voltage-dependent calcium channel
business.industry
Sodium channel
Sodium
Depolarization
Electric Stimulation
chemistry
Mice, Inbred DBA
Biophysics
Verapamil
Cardiology and Cardiovascular Medicine
business
medicine.drug
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....68779e574672258e21056a3658e0d278