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Interleukin-15 receptor α expression in inflammatory bowel disease patients before and after normalization of inflammation with infliximab
- Source :
- Immunology. 138(1)
- Publication Year :
- 2012
-
Abstract
- Summary Interleukin-15 (IL-15) is a pro-inflammatory cytokine thought to contribute to the inflammation in inflammatory bowel diseases (IBD). The specific receptor chain IL-15Rα can be expressed as a transmembranous signalling receptor, or can be cleaved by a disintegrin and metalloprotease domain 17 (ADAM17) into a neutralizing, soluble receptor (sIL-15Rα). The aim of this study is to evaluate the expression of IL-15Rα in ulcerative colitis (UC) and Crohn's disease (CD) patients before and after infliximab (IFX) therapy. Gene expression of IL-15Rα, IL-15 and ADAM17 was measured at the mRNA level by quantitative reverse transcription-PCR in mucosal biopsies harvested before and after first IFX therapy. Concentrations of sIL-15Rα were measured in sera of patients by ELISA and IL-15Rα protein was localized in the gut by immunohistochemistry and immunofluorescence. Mucosal expression of IL-15Rα is increased in UC and CD patients compared with controls and it remains elevated after IFX therapy in both responder and non-responder patients. The concentration of sIL-15Rα in serum is also increased in UC patients when compared with controls and does not differ between responders and non-responders either before or after IFX. CD patients have levels of sIL-15Rα comparable to healthy controls before and after therapy. In mucosal tissues, IL-15Rα+ cells closely resemble activated memory B cells with a pre-plasmablastic phenotype. To conclude, IBD patients have an increased expression of IL-15Rα mRNA in the mucosa. Expression is localized in B cells, suggesting that IL-15 regulates B-cell functions during bowel inflammation. No change in release of sIL-15Rα is observed in patients treated with IFX.
- Subjects :
- Adult
Male
medicine.medical_treatment
Immunology
Fluorescent Antibody Technique
Inflammation
Enzyme-Linked Immunosorbent Assay
Inflammatory bowel disease
Young Adult
Crohn Disease
Interleukin-15 Receptor alpha Subunit
medicine
Immunology and Allergy
Humans
RNA, Messenger
Colitis
business.industry
Reverse Transcriptase Polymerase Chain Reaction
Anti-Inflammatory Agents, Non-Steroidal
Antibodies, Monoclonal
Original Articles
Middle Aged
medicine.disease
Ulcerative colitis
Immunohistochemistry
Infliximab
Interleukin-15 receptor
Cytokine
Interleukin 15
Colitis, Ulcerative
Female
medicine.symptom
business
medicine.drug
Subjects
Details
- ISSN :
- 13652567
- Volume :
- 138
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Immunology
- Accession number :
- edsair.doi.dedup.....6861fece85cbffd2adc239b0497c4fc1