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DNMT1-Induced miR-152-3p Suppression Facilitates Cardiac Fibroblast Activation in Cardiac Fibrosis
- Source :
- Cardiovascular toxicology. 21(12)
- Publication Year :
- 2021
-
Abstract
- Novel insights into epigenetic control of cardiac fibrosis are now emerging. Cardiac fibroblasts (CFs) activation into myofibroblasts and the production of extracellular matrix (ECM) is the key to cardiac fibrosis development, but the specific mechanism is not fully understood. In the present study, we found that DNMT1 hypermethylation reduces the expression of microRNA-152-3p (miR-152-3p) and promotes Wnt1/β-catenin signaling pathway leading to CFs proliferation and activation. Cardiac fibrosis was produced by ISO, and the ISO was carried out according to the method described. CFs were harvested and cultured from SD neonatal rats and stimulated with TGF-β1. Importantly, DNMT1 resulted in the inhibition of miR-152-3p in activated CFs and both DNMT1 and miR-152-3p altered Wnt/β-catenin downstream protein levels. Over expression of DNMT1 and miR-152-3p inhibitors promotes proliferation of activating CFs. In addition, decreased methylation levels and over expression of miR-152-3p inhibited CFs proliferation. We determined that DNMT1 can methylate to miR-152-3p and demonstrated that expression of miR-152-3p inhibits CFs proliferation by inhibiting the Wnt1/β-catenin pathway. Our results stand out together DNMT1 methylation regulates miR-152-3p to slow the progression of cardiac fibrosis by inhibiting the Wnt1/β-catenin pathway.
- Subjects :
- DNA (Cytosine-5-)-Methyltransferase 1
Male
Cardiac fibrosis
Down-Regulation
Toxicology
Epigenesis, Genetic
Extracellular matrix
Rats, Sprague-Dawley
medicine
Animals
WNT1
Molecular Biology
Wnt Signaling Pathway
Cells, Cultured
Cell Proliferation
Chemistry
Myocardium
Wnt signaling pathway
DNA Methylation
Fibroblasts
medicine.disease
Fibrosis
Disease Models, Animal
MicroRNAs
Phenotype
embryonic structures
DNA methylation
DNMT1
Cancer research
Signal transduction
Cardiology and Cardiovascular Medicine
Cardiomyopathies
Myofibroblast
Subjects
Details
- ISSN :
- 15590259
- Volume :
- 21
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- Cardiovascular toxicology
- Accession number :
- edsair.doi.dedup.....6852cc69732f1b0ed1a312c888990093