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Expression and Clinical Significance of CacyBP/SIP in Pancreatic Cancer
- Source :
- Pancreatology. 8:470-477
- Publication Year :
- 2008
- Publisher :
- Elsevier BV, 2008.
-
Abstract
- Calcyclin-binding protein or Siah-1-interacting protein (CacyBP/SIP), a component of the ubiquitin-mediated proteolysis, could bind SKP1-CUL1-F box protein complex and participate in beta-catenin degradation, which was found to be related to the malignant phenotypes of gastric cancer and renal cancer. However, the role of CacyBP/SIP in pancreatic cancer progression still remains unclear. Therefore, the aim of the present study was to investigate the expression and clinical significance of CacyBP/SIP in pancreatic cancer.Immunohistochemistry was carried out on paraffin-embedded sections of pancreatic cancer and normal pancreatic tissues. In addition, Western blot and semiquantitative RT-PCR were carried out to analyze mRNA and protein expression of CacyBP/SIP in 8 pairs of freshly resected pancreatic cancer and their adjacent nontumorous tissue.CacyBP/SIP expression was significantly increased in pancreatic cancer tissue (28/68 or 41.2%) and correlated with differentiation degree, higher TNM (tumor, node, metastasis) stage and distance metastasis. Also, mRNA and protein expression of CacyBP/SIP were found to be at higher levels in almost all cancer tissues compared to adjacent tissues.CacyBP/SIP protein might play an important role in the process of pancreatic carcinogenesis and high-level CacyBP/SIP expression might be related to the malignant potential of pancreatic cancer.
- Subjects :
- Male
Oncology
medicine.medical_specialty
Endocrinology, Diabetes and Metabolism
Proteolysis
Blotting, Western
Gene Expression
Biology
medicine.disease_cause
Pancreatic cancer
Internal medicine
Gene expression
medicine
Humans
Clinical significance
Neoplasm Staging
Regulation of gene expression
Hepatology
medicine.diagnostic_test
Reverse Transcriptase Polymerase Chain Reaction
Calcium-Binding Proteins
Gastroenterology
Middle Aged
medicine.disease
Gene Expression Regulation, Neoplastic
Pancreatic Neoplasms
Blot
Cancer research
Female
CA19-9
Carcinogenesis
Subjects
Details
- ISSN :
- 14243903
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- Pancreatology
- Accession number :
- edsair.doi.dedup.....684458499feb6cc8afb5056aca189464