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Nonconcordant regulation of mitochondrial respiratory complexes in the kidneys of 5/6 nephrectomized mice
- Source :
- The Journal of Medical Investigation. 64:255-261
- Publication Year :
- 2017
- Publisher :
- University of Tokushima Faculty of Medicine, 2017.
-
Abstract
- Hyperglycemia induces nonconcordant regulation of renal mitochondrial respiratory complexes, increases oxidative stress, and causes diabetic nephropathy. Hypertension is a complication associated with diabetes and involves glomerular hyperfiltration, the effects of which on mitochondrial respiratory complexes are not well understood. To investigate the effect of glomerular hyperfiltration on renal mitochondrial respiratory complexes, we used the 5/6 nephrectomized BKS. Cg-Dock7m+/+Leprdb/J, Dock7m+/+Leprdb mice (db/m-5/6Nx mice) as a model for glomerular hyperfiltration. The BKS. Cg-Dock7m+/+Leprdb/J, +Leprdb/+Leprdb mice (db/db mice), a model for type 2 diabetes, was used as the positive control. We investigated the activities and protein levels of the mitochondrial complex, and the mitochondrial DNA and adenosine triphosphate content in the kidneys of these models. Blood chemistry and renal histopathological examination were performed for characterization of the disease. Both models showed expansion of the mesangial matrix of the glomeruli, which is indicative of glomerular hyperfiltration. The activities of complexes I and IV and the protein levels of complexes I and III were nonconcordant in db/m-5/6Nx mice. In conclusion, we demonstrated that nonconcordant regulation of mitochondrial complexes in db/m-5/6Nx mice involved with glomerular hyperfiltration. The progression and/or severity of nephropathy might be affected through a synergistic effect of mitochondrial dysfunction in hyperglycemia and glomerular hyperfiltration. J. Med. Invest. 64: 255-261, August, 2017.
- Subjects :
- Male
0301 basic medicine
Mitochondrial DNA
medicine.medical_specialty
Kidney
urologic and male genital diseases
medicine.disease_cause
Nephrectomy
General Biochemistry, Genetics and Molecular Biology
Nephropathy
Electron Transport Complex IV
Diabetic nephropathy
Mice
03 medical and health sciences
Internal medicine
Diabetes mellitus
medicine
Animals
Diabetic Nephropathies
Respiratory system
Electron Transport Complex I
Chemistry
General Medicine
medicine.disease
Mitochondria
030104 developmental biology
Endocrinology
Blood chemistry
Hyperglycemia
Glomerular hyperfiltration
Oxidative stress
Subjects
Details
- ISSN :
- 13496867 and 13431420
- Volume :
- 64
- Database :
- OpenAIRE
- Journal :
- The Journal of Medical Investigation
- Accession number :
- edsair.doi.dedup.....6842514acb2210d3e7d9fb3222e5f278