Supplementary Materials and Methods, Figure Legends, Figures 1 - 9, Tables 1 - 4 from Interleukin-32 Increases Human Gastric Cancer Cell Invasion Associated with Tumor Progression and Metastasis

PDF file - 1228KB, Supplementary Table SI. Primary antibody for western blot. Supplementary Table SII. Primary antibody for immunohistochemistry. Supplementary Table SIII. ShRNA for gene silencing. Supplementary Table SIV. Inhibitors for kinase inhibition. Supplemental Materials and Methods. Supplemental Figures and Legends. Supplementary Figure S1. IL-32 is overexpressing in GC specimens. Supplementary Figure S2. Ectopic overexpression of IL-32 promotes migration in TSGH9201 cells. Supplementary Figure S3. IL-32 enhances cell invasion and beta-catenin activity in a doxycycline-inducible expression system. Supplementary Figure S4. PI3K/AKT inhibitor (LY294002) diminishes IL-32-induced IL-8 expression. Supplementary Figure S5. Inhibition of phospho-AKT by MK-2206 attenuates expression of IL-32-induced downstream molecules as well as cell invasion ability. Supplementary Figure S6. Depletion of IL-32 in TSGH9201 and AGS cell lines. Supplementary Figure S7. Scatter plots of IL-32 and its downstream effectors in Spearman rank correlation analysis. Supplementary Figure S8. Rac GTPase signaling is partially involved in IL-32-induced cell migration. Supplementary Figure S9. HIF-1alpha mediated IL32-induced IL8, VEGF, MMP2 and MMP9 expression as well as cell invasion via a beta-catenin independent pathway.