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Bioelectronic DNA detection of human papillomaviruses using eSensor: a model system for detection of multiple pathogens

Authors :
Elizabeth R. Unger
Gary Blackburn
William C. Reeves
Suzanne D. Vernon
Daniel H. Farkas
Donna L. Miller
Vivian Chan
Yin-Peng Chen
Source :
BMC Infectious Diseases, BMC Infectious Diseases, Vol 3, Iss 1, p 12 (2003)
Publication Year :
2003

Abstract

Background We used human papillomaviruses (HPV) as a model system to evaluate the utility of a nucleic acid, hybridization-based bioelectronic DNA detection platform (eSensorâ„¢) in identifying multiple pathogens. Methods Two chips were spotted with capture probes consisting of DNA oligonucleotide sequences specific for HPV types. Electrically conductive signal probes were synthesized to be complementary to a distinct region of the amplified HPV target DNA. A portion of the HPV L1 region that was amplified by using consensus primers served as target DNA. The amplified target was mixed with a cocktail of signal probes and added to a cartridge containing a DNA chip to allow for hybridization with complementary capture probes. Results Two bioelectric chips were designed and successfully detected 86% of the HPV types contained in clinical samples. Conclusions This model system demonstrates the potential of the eSensor platform for rapid and integrated detection of multiple pathogens.

Details

ISSN :
14712334
Volume :
3
Database :
OpenAIRE
Journal :
BMC infectious diseases
Accession number :
edsair.doi.dedup.....680c54cb6611811e34d48d7e16279e3b