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Bioelectronic DNA detection of human papillomaviruses using eSensor: a model system for detection of multiple pathogens
- Source :
- BMC Infectious Diseases, BMC Infectious Diseases, Vol 3, Iss 1, p 12 (2003)
- Publication Year :
- 2003
-
Abstract
- Background We used human papillomaviruses (HPV) as a model system to evaluate the utility of a nucleic acid, hybridization-based bioelectronic DNA detection platform (eSensorâ„¢) in identifying multiple pathogens. Methods Two chips were spotted with capture probes consisting of DNA oligonucleotide sequences specific for HPV types. Electrically conductive signal probes were synthesized to be complementary to a distinct region of the amplified HPV target DNA. A portion of the HPV L1 region that was amplified by using consensus primers served as target DNA. The amplified target was mixed with a cocktail of signal probes and added to a cartridge containing a DNA chip to allow for hybridization with complementary capture probes. Results Two bioelectric chips were designed and successfully detected 86% of the HPV types contained in clinical samples. Conclusions This model system demonstrates the potential of the eSensor platform for rapid and integrated detection of multiple pathogens.
- Subjects :
- Microbiological Techniques
biology
Hybridization probe
virus diseases
Model system
Biosensing Techniques
biology.organism_classification
Virology
lcsh:Infectious and parasitic diseases
Dna detection
Infectious Diseases
Nucleic acid
Humans
Research article
lcsh:RC109-216
DNA Probes, HPV
Papillomaviridae
DNA Primers
Oligonucleotide Array Sequence Analysis
Research Article
Subjects
Details
- ISSN :
- 14712334
- Volume :
- 3
- Database :
- OpenAIRE
- Journal :
- BMC infectious diseases
- Accession number :
- edsair.doi.dedup.....680c54cb6611811e34d48d7e16279e3b