Enterococcus saccharolyticus subsp. taiwanensis subsp. nov., isolated from broccoli

A coccal strain isolated from fresh broccoli was initially identified asEnterococcus saccharolyticus; however, molecular identification and phenotypic traits did not support this identification. DNA–DNA hybridization with the type strain ofE. saccharolyticus(76.4 % relatedness), DNA G+C content (35.7 mol%), phylogenetic analysis based on 16S rRNA,pheSandrpoAgene sequences, rep-PCR fingerprinting and profiles of cellular fatty acids, whole-cell proteins and enzyme activities, together with carbohydrate metabolism characteristics, indicated that this strain is distinct and represents a novel subspecies, for which the nameEnterococcus saccharolyticussubsp.taiwanensissubsp. nov. is proposed. The type strain is 812T( = NBRC 109476T = BCRC 80575T). Furthermore, we present an emended description ofEnterococcus saccharolyticusand proposal ofEnterococcus saccharolyticussubsp.saccharolyticussubsp. nov. (type strain ATCC 43076T = CCUG 27643T = CCUG 33311T = CIP 103246T = DSM 20726T = JCM 8734T = LMG 11427T = NBRC 100493T = NCIMB 702594T).