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The Response of IL-17-Producing B Cells to ArtinM Is Independent of Its Interaction with TLR2 and CD14
- Source :
- Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, Molecules, Vol 23, Iss 9, p 2339 (2018), Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual), Universidade de São Paulo (USP), instacron:USP
- Publication Year :
- 2018
- Publisher :
- MDPI, 2018.
-
Abstract
- ArtinM, a d-mannose-binding lectin from Artocarpus heterophyllus, activates antigen-presenting cells by recognizing Toll-like receptor (TLR)2 and cluster of differentiation (CD)14 N-glycans, induces cytokine production, and promotes type 1 T helper (Th1) immunity, a process that plays an assisting role in the combat against fungal infections. We recently demonstrated that ArtinM stimulates CD4+ T cells to produce interleukin (IL)-17 through direct interaction with CD3. Here, we further investigated the effects of ArtinM on the production of IL-17 by B cell activation. We showed that ArtinM activates murine B cells, increasing IL-17 and IL-12p40 production. The direct effect of ArtinM was sufficient to induce IL-17 production in B cells, and we did not find differences in the levels of IL-17 between the B cells purified from the wild-type (WT) and knockout (KO) mice for TLR2 or CD14 in the presence of ArtinM. Thus, the effects of ArtinM on splenic B cells through carbohydrate recognition may contribute to Th17 immunity; however, the mechanism involved is not associated with the interaction of ArtinM with TLR2 and CD14. The current work represents a pioneering effort in the understanding of the induction of IL-17 by lectins in B cells.
- Subjects :
- 0301 basic medicine
REGULAÇÃO GÊNICA
CD14
CD3
medicine.medical_treatment
carbohydrate recognition
Lipopolysaccharide Receptors
Pharmaceutical Science
Lymphocyte Activation
Analytical Chemistry
lcsh:QD241-441
03 medical and health sciences
ArtinM
Gene Knockout Techniques
Mice
0302 clinical medicine
lcsh:Organic chemistry
Immunity
Drug Discovery
medicine
TLR2
Animals
Physical and Theoretical Chemistry
Cells, Cultured
B cells
B-Lymphocytes
Cluster of differentiation
biology
Chemistry
Communication
Organic Chemistry
Interleukin-17
Interleukin
Toll-Like Receptor 2
Cell biology
IL-17
030104 developmental biology
Cytokine
Gene Expression Regulation
Chemistry (miscellaneous)
biology.protein
Molecular Medicine
Interleukin 17
Plant Lectins
Artocarpus
030215 immunology
Subjects
Details
- Language :
- English
- ISSN :
- 14203049
- Volume :
- 23
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry
- Accession number :
- edsair.doi.dedup.....67e86de2b95c1259533c71571a44b750