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Membrane-bound Klotho is not expressed endogenously in healthy or uraemic human vascular tissue
- Source :
- Cardiovascular Research, 108, 220-31, Cardiovascular Research, 108(2), 220-231. Oxford University Press, Mencke, R, Harms, G, Mirkovic, K, Struik, J, van Ark, J, Van Loon, E, Verkaik, M, de Borst, M H, Zeebregts, C J, Hoenderop, J G, Vervloet, M G & Hillebrands, J L 2015, ' Membrane-bound Klotho is not expressed endogenously in healthy or uraemic human vascular tissue ', Cardiovascular Research, vol. 108, no. 2, pp. 220-231 . https://doi.org/10.1093/cvr/cvv187, Cardiovascular Research, 108(2), 220-231, Cardiovascular Research, 108, 2, pp. 220-31
- Publication Year :
- 2015
-
Abstract
- Contains fulltext : 152471.pdf (Publisher’s version ) (Closed access) AIMS: Cardiovascular disease (CVD) is the leading cause of death in patients with chronic kidney disease (CKD), a disease state that is strongly associated with loss of renal and systemic (alpha-)Klotho. Reversely, murine Klotho deficiency causes marked medial calcification. It is therefore thought that Klotho conveys a vasculoprotective effect. Klotho expression in the vessel wall, however, is disputed. METHODS AND RESULTS: We assessed Klotho expression in healthy human renal donor arteries (n = 9), CKD (renal graft recipient) arteries (n = 10), carotid endarterectomy specimens (n = 8), other elastic arteries (three groups of n = 3), and cultured human aortic smooth muscle cells (HASMCs) (three primary cell lines), using immunohistochemistry (IHC), immunofluorescence, quantitative reverse transcriptase-polymerase chain reaction, and western blotting (WB). We have extensively validated anti-Klotho antibody KM2076 by comparing staining patterns with other anti-Klotho antibodies (SC-22220, SC-22218, and AF1819), competition assays with recombinant Klotho, IHC on Klotho-deficient kl/kl mouse kidney, and WB with recombinant Klotho. Using KM2076, we could not detect full-length Klotho in vascular tissues or HASMCs. On the mRNA level, using primers against all four exon junctions, klotho expression could not be detected either. Fibroblast growth factor 23 (FGF23) injections in mice induced FGF23 signalling in kidneys but not in the aorta, indicating the absence of Klotho-dependent FGF23 signalling in the aorta. CONCLUSION: Using several independent and validated methods, we conclude that full-length, membrane-bound Klotho is not expressed in healthy or uraemic human vascular tissue.
- Subjects :
- Fibroblast growth factor 23
CHRONIC KIDNEY-DISEASE
medicine.medical_specialty
RENAL-FAILURE
Physiology
SMOOTH-MUSCLE-CELLS
PROTEIN
Biology
urologic and male genital diseases
Klotho
Physiology (medical)
medicine.artery
Internal medicine
Chronic kidney disease
medicine
CARDIOVASCULAR EVENTS
Kidney
Aorta
TRANSCRIPTS ENCODING MEMBRANE
medicine.disease
Cardiovascular disease
GENE
CALCIFICATION
female genital diseases and pregnancy complications
Blot
MICE
medicine.anatomical_structure
Endocrinology
Renal disorders Radboud Institute for Molecular Life Sciences [Radboudumc 11]
Smooth muscle cells
Immunohistochemistry
Cardiology and Cardiovascular Medicine
Kidney disease
Calcification
GROWTH-FACTOR 23
Subjects
Details
- ISSN :
- 00086363
- Database :
- OpenAIRE
- Journal :
- Cardiovascular Research, 108, 220-31, Cardiovascular Research, 108(2), 220-231. Oxford University Press, Mencke, R, Harms, G, Mirkovic, K, Struik, J, van Ark, J, Van Loon, E, Verkaik, M, de Borst, M H, Zeebregts, C J, Hoenderop, J G, Vervloet, M G & Hillebrands, J L 2015, ' Membrane-bound Klotho is not expressed endogenously in healthy or uraemic human vascular tissue ', Cardiovascular Research, vol. 108, no. 2, pp. 220-231 . https://doi.org/10.1093/cvr/cvv187, Cardiovascular Research, 108(2), 220-231, Cardiovascular Research, 108, 2, pp. 220-31
- Accession number :
- edsair.doi.dedup.....67d1889e7f5bd00e70811cc5a8627194