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Genome-wide analysis of HOXC4 and HOXC6 regulated genes and binding sites in prostate cancer cells
- Source :
- PLoS ONE, Vol 15, Iss 2, p e0228590 (2020), PLoS ONE
- Publication Year :
- 2020
- Publisher :
- Public Library of Science (PLoS), 2020.
-
Abstract
- Aberrant expression of HOXC6 and HOXC4 is commonly detected in prostate cancer. The high expression of these transcription factors is associated with aggressive prostate cancer and can predict cancer recurrence after treatment. Thus, HOXC4 and HOXC6 are clinically relevant biomarkers of aggressive prostate cancer. However, the molecular mechanisms by which these HOXC genes contribute to prostate cancer is not yet understood. To begin to address the role of HOXC4 and HOXC6 in prostate cancer, we performed RNA-seq analyses before and after siRNA-mediated knockdown of HOXC4 and/or HOXC6 and also performed ChIP-seq to identify genomic binding sites for both of these transcription factors. Our studies demonstrate that HOXC4 and HOXC6 co-localize with HOXB13, FOXA1 and AR, three transcription factors previously shown to contribute to the development of prostate cancer. We suggest that the aberrantly upregulated HOXC4 and HOXC6 proteins may compete with HOXB13 for binding sites, thus altering the prostate transcriptome. This competition model may be applicable to many different human cancers that display increased expression of a HOX transcription factor.
- Subjects :
- 0301 basic medicine
Male
Biochemistry
Transcriptome
Prostate cancer
0302 clinical medicine
Prostate
Medicine and Health Sciences
Small interfering RNAs
Regulatory Elements, Transcriptional
Regulation of gene expression
Gene knockdown
Multidisciplinary
Chromosome Biology
Prostate Cancer
Prostate Diseases
Chromatin
3. Good health
Gene Expression Regulation, Neoplastic
Nucleic acids
medicine.anatomical_structure
Oncology
030220 oncology & carcinogenesis
Medicine
Epigenetics
Anatomy
Protein Binding
Research Article
Transcriptional Activation
Urology
Science
Biology
03 medical and health sciences
Exocrine Glands
Protein Domains
Cell Line, Tumor
DNA-binding proteins
medicine
Genetics
Humans
Homeobox
Non-coding RNA
Transcription factor
Gene
Homeodomain Proteins
Prostatic Neoplasms
Cancers and Neoplasms
Biology and Life Sciences
Proteins
Cell Biology
medicine.disease
Gene regulation
Regulatory Proteins
Genitourinary Tract Tumors
030104 developmental biology
Cancer research
RNA
Prostate Gland
Gene expression
FOXA1
Transcription Factors
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 15
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....67b167cf58c4409572569ff86c17f6f6