Optimization of cello-oligosaccharides production by enzymatic hydrolysis of hydrothermally pretreated sugarcane straw using cellulolytic and oxidative enzymes

Made available in DSpace on 2020-12-12T01:33:52Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-10-01 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Enzymatic hydrolysis of lignocellulosic biomass accounts for 20–30% of the total cost of second-generation bioethanol production and many efforts have been made in recent years to overcome the high cost of enzymes. Using cello-oligosaccharides (COS), intermediates in cellulose conversion to glucose, may provide advantages over monomeric glucose fermentation, such as lower risk of growth of process contaminants, shorter fermentation time and limited process inhibition by high concentrations of glucose. In addition, COS are also useful as functional oligosaccharides in the food and feed sectors. This study aimed to optimize COS production for further industrial applications. To the best of our knowledge, this is the first study that has used a design of experiments approach to analyze the synergism between endoglucanases, lytic polysaccharide monooxygenase (LPMO), cellobiose dehydrogenase (CDH) and different additives during the hydrolysis of a pretreated sugarcane straw for COS production. After optimization of enzymatic hydrolysis, a combination of the endoglucanases CaCel and CcCel9m, the LPMO TrCel61A, the CDH NcCDHIIa, with lactose and copper as additives, produced 60.49 mg of COS per g of pretreated sugarcane straw, 1.8–2.7-fold more than the commercial enzyme cocktails Cellic® Ctec2 and Celluclast® 1.5 L. The COS/glucose ratio achieved was 298.31, an increase of 3314 and 2294-fold over the commercial enzymatic cocktails, respectively. These results open a new perspective regarding COS production and its industrial application. Bioprocess and Metabolic Engineering Laboratory School of Food Engineering University of Campinas (UNICAMP) Department of Biology & Biochemistry Faculty of Sciences University of Bath Interdisciplinary Center of Energy Planning University of Campinas Brazilian Biorenewables National Laboratory (LNBR) Brazilian Center for Research in Energy and Materials (CNPEM) Bioflavors and Bioactive Compounds Laboratory Department of Food Science School of Food Engineering University of Campinas Department of Bioprocess and Biotechnology College of Agricultural Sciences São Paulo State University (UNESP) Department of Biochemistry and Tissue Biology Institute of Biology University of Campinas (UNICAMP) Laboratory of Biochemical Engineering Biorefining and Products of Renewable Origin (LEBBPOR) Faculty of Chemical Engineering University of Campinas (UNICAMP) Department of Bioprocess and Biotechnology College of Agricultural Sciences São Paulo State University (UNESP)