Cytotoxicity of chlorhexidine on human osteoblastic cells is related to intracellular glutathione levels

Lee T-H, Hu C-C, Lee S-S, Chou M-Y, Chang Y-C. Cytotoxicity of chlorhexidine on human osteoblastic cells is related to intracellular glutathione levels. International Endodontic Journal, 43, 430–435, 2010. Abstract Aim To evaluate the mechanisms of cytotoxicity of chlorhexidine (CHX) in human osteoblastic cells in vitro. Methodology Cytotoxicity, cell proliferation and collagen synthesis assays were performed to elucidate the toxic effects of CHX on the human osteoblastic cell line U2OS. To determine whether glutathione (GSH) levels were important in the cytotoxicity of CHX, cells were pre-treated with 2-oxothiazolidine-4-carboxylic acid (OTZ) to boost GSH levels or buthionine sulfoximine (BSO) to deplete GSH. Results CHX demonstrated a cytotoxic effect to U2OS cells in a dose-dependent manner (P