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Semi-quantitative immunohistochemical detection of 5-hydroxymethyl-cytosine reveals conservation of its tissue distribution between amphibians and mammals
- Source :
- Almeida, R D, Sottile, V, Loose, M, De Sousa, P A, Johnson, A D & Ruzov, A 2012, ' Semi-quantitative immunohistochemical detection of 5-hydroxymethyl-cytosine reveals conservation of its tissue distribution between amphibians and mammals ', Epigenetics, vol. 7, no. 2, pp. 137-140 . https://doi.org/10.4161/epi.7.2.18949
- Publication Year :
- 2012
- Publisher :
- Taylor & Francis Open, 2012.
-
Abstract
- 5-Hydroxymethyl-cytosine (5-hmC) is a form of modified cytosine, which has recently attracted a considerable attention due to its potential role in transcriptional regulation. According to several reports 5-hydroxymethyl-cytosine distribution is tissue-specific in mammals. Thus, 5-hmC is enriched in embryonic cell populations and in adult neuronal tissue. Here, we describe a novel method of semi-quantitative immunohistochemical detection of 5-hmC and utilize it to assess the levels of this modification in amphibian tissues. We show that, similar to mammalian embryos, 5-hmC is enriched in axolotl tadpoles compared with adult tissues. Our data demonstrate that 5-hmC distribution is tissue-specific in amphibians, and that strong 5-hmC enrichment in neuronal cells is conserved between amphibians and mammals. In addition, we identify 5-hmC-enriched cell populations that are distributed in amphibian skin and connective tissue in a mosaic manner. Our results illustrate that immunochemistry can be successfully used not only for spatial identification of cells enriched with 5-hmC, but also for the semi-quantitative assessment of the levels of this epigenetic modification in single cells of different tissues.
- Subjects :
- Amphibian
Cancer Research
Xenopus
Connective tissue
5-methyl-cytosine
Amphibians
semi-quantitative immunohistochemistry
Cytosine
Mice
chemistry.chemical_compound
Axolotl
biology.animal
evolution
medicine
Animals
Humans
Tissue Distribution
Epigenetics
Molecular Biology
Zebrafish
Phylogeny
Mammals
5-Hydroxymethylcytosine
epigenetics
biology
Brief Report
5-hydroxymethyl-cytosine
DNA
biology.organism_classification
Immunohistochemistry
Embryonic stem cell
Molecular biology
Cell biology
Ambystoma mexicanum
DNA-Binding Proteins
CONVERSION
5-Methylcytosine
medicine.anatomical_structure
chemistry
regeneration
5-HYDROXYMETHYLCYTOSINE
Chickens
Subjects
Details
- Language :
- English
- ISSN :
- 15592294
- Database :
- OpenAIRE
- Journal :
- Almeida, R D, Sottile, V, Loose, M, De Sousa, P A, Johnson, A D & Ruzov, A 2012, ' Semi-quantitative immunohistochemical detection of 5-hydroxymethyl-cytosine reveals conservation of its tissue distribution between amphibians and mammals ', Epigenetics, vol. 7, no. 2, pp. 137-140 . https://doi.org/10.4161/epi.7.2.18949
- Accession number :
- edsair.doi.dedup.....6780194e02ce8f5adb546d8baa533cea