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5' and 3' splicing signals evolution in vertebrates: Analysis in a conserved gene family
- Source :
- Computational biology and chemistry. 86
- Publication Year :
- 2019
-
Abstract
- The mitochondrial solute carrier genes (SLC25) are highly conserved during vertebrate evolution. In most SLC25 genes of zebrafish, chicken, mouse, and human, the introns are located at exactly superimposable positions. In these topographically corresponding introns we studied the composition of the initial and terminal hexanucleotides (5’ss and 3’ss) which are instrumental in splicing signaling, focusing on the evolutionary conservation/mutation dynamics of these genetically related sequences. At each position, the per cent conservation of zebrafish individual nucleotides in chicken, mouse and human is proportional to their percent frequency in zebrafish; furthermore, nucleotide mutations are biased in favor of the more represented nucleotides, thus compensating for those highly represented zebrafish nucleotides which have not been conserved. As a result of these evolutionary dynamics, the general nucleotide composition at each position has remained relatively conserved throughout vertebrates. At 5’ss, following the canonical GT, A and G are largely prevailing at position +3, A at +4 and G at +5 (GT[A/G]AGx). At 3’ss, T and C are largely prevailing at positions −6, −5 and −3, preceding the canonical intron terminal AG ([C/T] [C/T]x[C/T]AG). However, the actual composition of the tetranucleotides at 5’ and 3’ often does not conform to the above scheme. At 5’ss the more canonical sequence is completely expressed in 63% of cases and partially (2 or 1 matches) in 37 % of cases. At 3’ss the more canonical sequence is completely expressed in 71 % of cases and partially (2 or 1 matches) in 29 % of cases. The nucleotide conservation loss (nucleotide mutation) is higher in the evolution from fish to the last common ancestor of birds and mammals (58 %), then diminishes in the successive evolution steps up to the mammalian common ancestor (10 %), and becomes still lower at the divergence of rodents and primates (5 %).
- Subjects :
- 0301 basic medicine
Most recent common ancestor
RNA Splicing
Organic Anion Transporters
Biology
medicine.disease_cause
Biochemistry
Conserved sequence
Evolution, Molecular
Mitochondrial Proteins
03 medical and health sciences
Mice
0302 clinical medicine
Structural Biology
medicine
Gene family
Animals
Humans
Zebrafish
Gene
Conserved Sequence
Genetics
Mutation
Base Sequence
Organic Chemistry
Intron
biology.organism_classification
Introns
Computational Mathematics
030104 developmental biology
030220 oncology & carcinogenesis
RNA splicing
RNA Splice Sites
Chickens
Subjects
Details
- ISSN :
- 1476928X
- Volume :
- 86
- Database :
- OpenAIRE
- Journal :
- Computational biology and chemistry
- Accession number :
- edsair.doi.dedup.....676f83f93ba964aa8417e1355882babb