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Hybridomas for production of monoclonal antibodies to human erythropoietin
- Source :
- Europe PubMed Central
- Publication Year :
- 1984
- Publisher :
- American Society of Hematology, 1984.
-
Abstract
- Human urinary erythropoietin has been highly purified by a combination of conventional purification methods and immunoadsorbent columns packed with hybridoma-produced antibodies against contaminants that seemed difficult to separate from erythropoietin by the usual means. By using the partially purified erythropoietin as an antigen, three hybridoma clones have been obtained that secrete monoclonal antibodies against erythropoietin. One of the clones has been quite stable, with a rapid growth rate and high production of antibody. Western blotting technique with monoclonal antibodies revealed occurrence of two species of erythropoietin. The monoclonal antibody will be useful as a probe for the purification of erythropoietin and for further studies of the hormone and its mechanism of action.
- Subjects :
- Paper
medicine.drug_class
Immunology
Monoclonal antibody
Biochemistry
Antigen-Antibody Reactions
Mice
Antigen
hemic and lymphatic diseases
medicine
Animals
Humans
Secretion
Purification methods
Erythropoietin
Immunosorbent Techniques
Mice, Inbred BALB C
Hybridomas
biology
Chemistry
Antibodies, Monoclonal
Collodion
Cell Biology
Hematology
Molecular biology
Blot
Mechanism of action
biology.protein
Electrophoresis, Polyacrylamide Gel
Female
Antibody
medicine.symptom
Drug Contamination
medicine.drug
Subjects
Details
- ISSN :
- 15280020 and 00064971
- Volume :
- 64
- Database :
- OpenAIRE
- Journal :
- Blood
- Accession number :
- edsair.doi.dedup.....6763763768caff62d00a277b32ecad4e