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Development of pH-responsive chitosan/heparin nanoparticles for stomach-specific anti-Helicobacter pylori therapy
- Source :
- Biomaterials. 30:3332-3342
- Publication Year :
- 2009
- Publisher :
- Elsevier BV, 2009.
-
Abstract
- The microorganism now known as Helicobacter pylori is considered to be an important factor in the etiology of peptic ulcers. It can secrete urease enzyme and buffer gastric acids to survive in the stomach. H. pylori can colonize the gastric mucosa and preferentially adheres near the cell-cell junctions of the gastric mucous cells. In this study, pH-responsive nanoparticles were produced instantaneously upon the addition of heparin solution to a chitosan solution with magnetic stirring at room temperature. The nanoparticles appeared to have a particle size of 130-300 nm, with a positive surface charge, and were stable at pH 1.2-2.5, allowing them to protect an incorporated drug from destructive gastric acids. We also demonstrated that the prepared nanoparticles can adhere to and infiltrate cell-cell junctions and interact locally with H. pylori infection sites in intercellular spaces.
- Subjects :
- Male
Materials science
Peptic
Biophysics
Biocompatible Materials
Bioengineering
Helicobacter Infections
Microbiology
Biomaterials
Chitosan
Mice
chemistry.chemical_compound
Materials Testing
Gastric mucosa
medicine
Animals
Humans
Secretion
Cells, Cultured
Fluorescent Dyes
Mice, Inbred BALB C
Helicobacter pylori
Molecular Structure
biology
Heparin
Stomach
Hydrogen-Ion Concentration
biology.organism_classification
Coculture Techniques
digestive system diseases
medicine.anatomical_structure
chemistry
Gastric Mucosa
Mechanics of Materials
Ceramics and Composites
Nanoparticles
Intracellular
medicine.drug
Subjects
Details
- ISSN :
- 01429612
- Volume :
- 30
- Database :
- OpenAIRE
- Journal :
- Biomaterials
- Accession number :
- edsair.doi.dedup.....67483caac4398cbfc53c0ecdd5b95a74