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Modulation of acute visceral nociception and bladder inflammation by plant triterpene, alpha, beta-amyrin in a mouse model of cystitis: role of tachykinin NK(1)-receptors, and K(+)(ATP) channels
- Source :
- Europe PubMed Central
-
Abstract
- We previously described the visceral antinociceptive property of alpha, beta-amyrin in a mouse model of cystitis induced by cyclophosphamide (CPM). This study examined the contribution of vanilloid-1 (TRPV1), peripheral NK1 receptors to CPM-evoked nociceptive behaviors and bladder edema, and its possible modulation by alpha, beta-amyrin.The effect of alpha, beta-amyrin (10, 30, and 100 mg/kg, p. o.) and N-acetylcysteine (NAC) on CPM (400 mg/kg, i. p.)-induced cystitis was studied in mice. Sensory deafferentation was done by a high dose capsaicin. The parameters analysed were: CPM-evoked noxious behaviors, bladder edema, vascular permeability, and NK(1) immunoreactivity. To assess the role of K(+) (ATP) channels in alpha, beta-amyrin effect, animals were pretreated with glibenclamide.alpha, beta-amyrin (30 and 100 mg/kg) and NAC significantly (p0.01) suppressed the visceral pain-related behaviors and NK(1) immunoreactivity, but bladder edema was reduced weakly. Glibenclamide reversed the effects of alpha, beta-amyrin. Sensory deafferentation by capsaicin significantly reduced the nociceptive responses and the NK(1) immunoreactivity to noxious stimulation by CPM.alpha, beta-amyrin attenuates CPM-induced visceral pain and bladder edema by mechanisms that involve, at least in part, a block either of Substance P release or its receptor function, and partly by opening K(+) (ATP) channels.
- Subjects :
- Male
Amyrin
Cyclophosphamide
Immunology
Urinary Bladder
TRPV1
Pain
TRPV Cation Channels
Pharmacology
Bladder inflammation
chemistry.chemical_compound
Mice
Triterpene
KATP Channels
parasitic diseases
Cystitis
Glyburide
Medicine
Animals
Edema
Oleanolic Acid
Receptor
chemistry.chemical_classification
business.industry
Anti-Inflammatory Agents, Non-Steroidal
Receptors, Neurokinin-1
Peripheral
Acetylcysteine
Nociception
chemistry
Anesthesia
embryonic structures
Capsaicin
business
human activities
medicine.drug
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Europe PubMed Central
- Accession number :
- edsair.doi.dedup.....674391778dfea88df4ab05ec728657f2