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Electroacupuncture Regulates Hippocampal Synaptic Plasticity via miR-134-Mediated LIMK1 Function in Rats with Ischemic Stroke
- Source :
- Neural Plasticity, Neural Plasticity, Vol 2017 (2017)
- Publication Year :
- 2017
- Publisher :
- Hindawi Publishing Corporation, 2017.
-
Abstract
- MircoRNAs (miRs) have been implicated in learning and memory, by regulating LIM domain kinase (LIMK1) to induce synaptic-dendritic plasticity. The study aimed to investigate whether miRNAs/LIMK1 signaling was involved in electroacupuncture- (EA-) mediated synaptic-dendritic plasticity in a rat model of middle cerebral artery occlusion induced cognitive deficit (MICD). Compared to untreatment or non-acupoint-EA treatment, EA at DU20 and DU24 acupoints could shorten escape latency and increase the frequency of crossing platform in Morris water maze test. T2-weighted imaging showed that the MICD rat brain lesions were located in cortex, hippocampus, corpus striatum, and thalamus regions and injured volumes were reduced after EA. Furthermore, we found that the density of dendritic spine and the number of synapses in the hippocampal CA1 pyramidal cells were obviously reduced at Day 14 after MICD. However, synaptic-dendritic loss could be rescued after EA. Moreover, the synaptic-dendritic plasticity was associated with increases of the total LIMK1 and phospho-LIMK1 levels in hippocampal CA1 region, wherein EA decreased the expression of miR-134, negatively regulating LIMK1 to enhance synaptic-dendritic plasticity. Therefore, miR-134-mediated LIMK1 was involved in EA-induced hippocampal synaptic plasticity, which served as a contributor to improving learning and memory during the recovery stage of ischemic stroke.
- Subjects :
- Male
0301 basic medicine
Dendritic spine
Article Subject
Dendritic Spines
Morris water navigation task
Hippocampus
Striatum
LIMK1
Hippocampal formation
Biology
lcsh:RC321-571
Brain Ischemia
Rats, Sprague-Dawley
Brain ischemia
03 medical and health sciences
0302 clinical medicine
Neuroplasticity
medicine
Animals
Cognitive Dysfunction
Maze Learning
lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry
CA1 Region, Hippocampal
Neuronal Plasticity
Pyramidal Cells
Brain
Lim Kinases
medicine.disease
Rats
Stroke
MicroRNAs
Electroacupuncture
030104 developmental biology
Neurology
Synapses
Neurology (clinical)
Neuroscience
030217 neurology & neurosurgery
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 20905904
- Database :
- OpenAIRE
- Journal :
- Neural Plasticity
- Accession number :
- edsair.doi.dedup.....672ee9c59e7ba825efdeecc9173c6443
- Full Text :
- https://doi.org/10.1155/2017/9545646