Back to Search
Start Over
Marine-derived fungus Aspergillus cf. tubingensis LAMAI 31: a new genetic resource for xylanase production
- Source :
- Scopus, Repositório Institucional da UNESP, Universidade Estadual Paulista (UNESP), instacron:UNESP, AMB Express
- Publisher :
- Springer Nature
-
Abstract
- Made available in DSpace on 2018-12-11T16:41:33Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-12-01 Marine-derived fungi have been reported as relevant producers of enzymes, which can have different properties in comparison with their terrestrial counterparts. The aim of the present study was to select from a collection of 493 marine-derived fungi the best producer of xylanase in order to evaluate the enzymatic production under different conditions. A total of 112 isolates produced xylanase in solid medium containing xylan as the carbon source, with 31 of them able to produce at least 10 U/mL of the enzyme. The best production (49.41 U/mL) was achieved by the strain LAMAI 31, identified as Aspergillus cf. tubingensis. After confirming the lack of pathogenicity (absence of ochratoxin A and fumonisin B2 production) this fungus was submitted to the experimental design in order to evaluate the effect of different variables on the enzymatic production, with the aim of optimizing culture conditions. Three experimental designs (two Plackett–Burman and one factorial fractional) were applied. The best condition for the enzymatic production was defined, resulting in an increase of 12.7 times in comparison with the initial production during the screening experiments. In the validation assay, the peak of xylanase production (561.59 U/mL) was obtained after 96 h of incubation, being the best specific activity achieved after 72 h of incubation. Xylanase from A. cf. tubingensis LAMAI 31 had optimum pH and temperature at 5.0 and 55 °C, respectively, and was shown to be stable at a range of 40–50 °C, and in pH from 3.6 to 7.0. Results from the present work indicate that A. cf. tubingensis LAMAI 31 can be considered as a new genetic resource for xylanase production. Departamento de Bioquímica e Microbiologia Universidade Estadual Paulista Júlio de Mesquita Filho—UNESP, 24A, 1515 Instituto de Tecnologia de Alimentos—ITAL Departamento de Bioquímica e Microbiologia Universidade Estadual Paulista Júlio de Mesquita Filho—UNESP, 24A, 1515
- Subjects :
- 0106 biological sciences
0301 basic medicine
Ochratoxin A
Biophysics
Fungus
Biology
01 natural sciences
Applied Microbiology and Biotechnology
Marine-derived fungi
03 medical and health sciences
chemistry.chemical_compound
010608 biotechnology
Botany
Marine biotechnology
Food science
Incubation
Fumonisin B2
Aspergillus
Strain (chemistry)
Xylanase
biology.organism_classification
Experimental design
030104 developmental biology
chemistry
Specific activity
Original Article
Subjects
Details
- Language :
- English
- ISSN :
- 21910855
- Volume :
- 6
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- AMB Express
- Accession number :
- edsair.doi.dedup.....672a2045b02eb93c0147c578182cff72
- Full Text :
- https://doi.org/10.1186/s13568-016-0194-z