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On a stake-out: Mycobacterial small RNA identification and regulation
- Source :
- Non-coding RNA Research, Non-coding RNA Research, Vol 4, Iss 3, Pp 86-95 (2019)
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- Persistence of mycobacteria in the hostile environment of human macrophage is pivotal for its successful pathogenesis. Rapid adaptation to diverse stresses is the key aspect for their survival in the host cells. A range of heterogeneous mechanisms operate in bacteria to retaliate stress conditions. Small RNAs (sRNA) have been implicated in many of those mechanisms in either a single or multiple regulatory networks to post-transcriptionally modulate bacterial gene expression. Although small RNA profiling in mycobacteria by advanced technologies like deep sequencing, tilling microarray etc. have identified hundreds of sRNA, however, a handful of those small RNAs have been unearthed with precise regulatory mechanism. Extensive investigations on sRNA-mediated gene regulations in eubacteria like Escherichia coli revealed the existence of a plethora of distinctive sRNA mechanisms e.g. base pairing, protein sequestration, RNA decoy etc. Increasing studies on mycobacterial sRNA also discovered several eccentric mechanisms where sRNAs act at the posttranscriptional stage to either activate or repress target gene expression that lead to promote mycobacterial survival in stresses. Several intrinsic features like high GC content, absence of any homologue of abundant RNA chaperones, Hfq and ProQ, isolate sRNA mechanisms of mycobacteria from that of other bacteria. An insightful approach has been taken in this review to describe sRNA identification and its regulations in mycobacterial species especially in Mycobacterium tuberculosis. Keywords: Small RNAs, Base pairing, Antisense, Anti-antisense, Gene regulation by sRNA
- Subjects :
- nt, nucleotide
0301 basic medicine
TILLING
Small RNA
TF, transcription factor
lcsh:QH426-470
Base pairing
Computational biology
Biology
Biochemistry
Article
TIR, translation initiation region
RNAP, RNA polymerase
Deep sequencing
03 medical and health sciences
0302 clinical medicine
ORF, open reading frame
Gene expression
Genetics
Antisense
Anti-antisense
Molecular Biology
Gene
Small RNAs
Biochemistry (medical)
SD, Shine Dalgarno sequence
CDS, coding sequence
RNA
sRNA, small RNA
lcsh:Genetics
UTR, untranslated region
IGR, intergenic region
030104 developmental biology
030220 oncology & carcinogenesis
Transfer RNA
RBS, Ribosome binding site
Gene regulation by sRNA
GC-content
Subjects
Details
- ISSN :
- 24680540
- Volume :
- 4
- Database :
- OpenAIRE
- Journal :
- Non-coding RNA Research
- Accession number :
- edsair.doi.dedup.....671f3ccacad6b49decbf4a75b5c66b8a