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Proteomic profiles and cytokeratin 13 as a potential biomarker of Ovis aries papillomavirus 3-positive and negative cutaneous squamous cell carcinomas
- Source :
- Research in Veterinary Science. 134:112-119
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- none 9 no Ovis aries papillomavirus 3 (OaPV3) is an epidermotropic PV reported in sheep cutaneous squamous cell carcinoma (SCC). The presence of OaPV3 DNA and its transcriptional activity in cutaneous SCC, as well as its in vitro transforming properties, suggest a viral etiology for this neoplasm. Nevertheless, the reactome associated with viral-host interaction is still unexplored. Here, we investigated and compared the proteomic profiles of OaPV3-positive SCCs, OaPV3-negative SCCs, and non-SCC samples by liquid chromatography tandem-mass spectrometry (LC-MS/MS) analysis, bioinformatics tools, and immunohistochemistry (IHC). OaPV3-positive SCCs (n = 3), OaPV3-negative SCCs (n = 3), and non-SCCs samples (n = 3) were subjected to a shotgun proteomic analysis workflow to assess protein abundance differences among the three sample classes. Proteins involved in epithelial cell differentiation, extracellular matrix organization, and apoptotic signaling showed different abundances in OaPV3-positive SCCs tissues (P ≤ 0.05) when compared to the other tissues. Cytokeratin 13 (CK 13) was among the most increased proteins in OaPV3-positive SCC and was validated by immunohistochemistry on 10 samples per class, confirming its potential as a biomarker of OaPV3 infection in SCC. Collectively, results provide a preliminary insight into the reactome associated with viral-host interaction and pave the way to the development of specific biomarkers for viral-induced sheep SCC. none Vitiello V.; Burrai G.P.; Pisanu S.; Cacciotto C.; Addis M.F.; Alberti A.; Antuofermo E.; Cubeddu T.; Pirino S. Vitiello V.; Burrai G.P.; Pisanu S.; Cacciotto C.; Addis M.F.; Alberti A.; Antuofermo E.; Cubeddu T.; Pirino S.
- Subjects :
- Skin Neoplasms
Proteome
Cell
Reactome
0403 veterinary science
Tandem Mass Spectrometry
Squamous cell carcinoma
Viral
Papillomaviridae
Domestic
Epithelial cell differentiation
Chromatography
Liquid
0303 health sciences
Tumor
biology
04 agricultural and veterinary sciences
Papillomavirus
medicine.anatomical_structure
Carcinoma, Squamous Cell
Biomarker (medicine)
Immunohistochemistry
Extracellular matrix organization
040301 veterinary sciences
Sheep Diseases
03 medical and health sciences
Cytokeratin
Cytokeratin 13
Sheep
Animals
Biomarkers, Tumor
Chromatography, Liquid
DNA, Viral
Keratin-13
Papillomavirus Infections
Sheep, Domestic
medicine
neoplasms
030304 developmental biology
General Veterinary
Carcinoma
DNA
biology.organism_classification
stomatognathic diseases
Squamous Cell
Cancer research
Biomarkers
Subjects
Details
- ISSN :
- 00345288
- Volume :
- 134
- Database :
- OpenAIRE
- Journal :
- Research in Veterinary Science
- Accession number :
- edsair.doi.dedup.....6719f285dd96d39b34ca6929bcd55be5