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Evolutionary Pathway Determines the Stoichiometric Response of Escherichia coli Adapted to High Temperature
- Source :
- Frontiers in Ecology and Evolution, Vol 5 (2018)
- Publication Year :
- 2018
- Publisher :
- Frontiers Media S.A., 2018.
-
Abstract
- Microorganisms exhibit shifts in elemental stoichiometry in response to short-term temperature increases due to varying growth rate, biochemical reactions, and protein degradation. Yet, it is unknown how an organism’s elemental stoichiometry will respond to temperature change on evolutionary timescales. Here we ask how cellular elemental stoichiometry and physiology change in Escherichia coli that have adapted to high temperature over 2,000 generations compared to their low temperature adapted ancestor. Cell lines evolved to a temperature of 42.2°C via two, negatively epistatic adaptive pathways leading to mutations in either the RNA polymerase complex or the termination factor rho. Compared to the ancestor, high temperature adapted cell lines overall had 14% higher N:P ratios, but did not differ significantly in C:N or C:P. However, cell lines with mutations in the rho gene had 13% lower C:N and 34% higher N:P. Furthermore, the two adaptive strategies of the rho and RNA polymerase mutations varied significantly from one another, cell lines with the rho mutation had lower C:N, higher N:P and higher protein content compared to cell lines with the RNA polymerase mutation. Thus, specific adaptive pathways modulate the effect of temperature on the cellular elemental stoichiometry and may explain why the elemental composition of specific lineages is differentially affected by temperature changes.
- Subjects :
- 0301 basic medicine
Termination factor
lcsh:Evolution
RNA polymerase complex
adaptation
Protein degradation
medicine.disease_cause
03 medical and health sciences
chemistry.chemical_compound
RNA polymerase
lcsh:QH540-549.5
medicine
Escherichia coli
lcsh:QH359-425
experimental evolution
Gene
Ecology, Evolution, Behavior and Systematics
Experimental evolution
Mutation
Ecology
Chemistry
temperature
stoichiometry
030104 developmental biology
Biochemistry
lcsh:Ecology
Subjects
Details
- Language :
- English
- Volume :
- 5
- Database :
- OpenAIRE
- Journal :
- Frontiers in Ecology and Evolution
- Accession number :
- edsair.doi.dedup.....670b37aab97b8effce9258fa8290cc63