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Development of anR-Selective Amine Oxidase with Broad Substrate Specificity and High Enantioselectivity
- Source :
- ChemCatChem
- Publication Year :
- 2014
- Publisher :
- Wiley, 2014.
-
Abstract
- Amine oxidases are useful bio-catalysts for the synthesis of enantiomerically pure 1°, 2° and 3° chiral amines. Enzymes in this class (e.g., MAO-N from Aspergillus niger) reported previously have been shown to be highly S selective. Herein we report the development of an enantiocomplementary R-selective amine oxidase based on 6-hydroxy-D-nicotine oxidase (6-HDNO) with broadened substrate scope and high enantioselectivity. The engineered 6-HDNO enzyme has been applied to the preparative deracemisation of a range of racemic amines to yield S-configured products, for example, (S)-nicotine, in high ee.
- Subjects :
- chemistry.chemical_classification
Amine oxidase
Oxidase test
biology
010405 organic chemistry
Organic Chemistry
Aspergillus niger
Substrate (chemistry)
010402 general chemistry
biology.organism_classification
01 natural sciences
Catalysis
0104 chemical sciences
Inorganic Chemistry
Enzyme
chemistry
Biocatalysis
Yield (chemistry)
Organic chemistry
Amine gas treating
Physical and Theoretical Chemistry
Subjects
Details
- ISSN :
- 18673880
- Volume :
- 6
- Database :
- OpenAIRE
- Journal :
- ChemCatChem
- Accession number :
- edsair.doi.dedup.....6701ead96d084325daf5e052053aec1f