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The Feasibility of Pressurised Intraperitoneal Aerosolised Virotherapy (PIPAV) to Administer Oncolytic Adenoviruses
- Source :
- PHARMACEUTICS, Pharmaceutics, Pharmaceutics; Volume 13; Issue 12; Pages: 2043, Pharmaceutics, Vol 13, Iss 2043, p 2043 (2021)
- Publication Year :
- 2021
- Publisher :
- MDPI AG, 2021.
-
Abstract
- Background: The prognosis of patients with peritoneal metastases is poor. Treatment options are limited because systemically delivered chemotherapy is not usually effective in this type of disease. Pressurised intraperitoneal aerosolised chemotherapy (PIPAC) is a recently developed alternative technology for delivering intraperitoneal chemotherapy, potentially enhancing treatment efficacy. Here, we assess the feasibility of pressurised intraperitoneal aerosolised virotherapy (PIPAV) to deliver a different class of anticancer agents, oncolytic adenoviruses, in vitro and in vivo. Methods: Adenoviral vectors expressing reporter genes green fluorescence protein (Ad5.GFP) or firefly luciferase (Ad5.Luc) were subject to pressurised aerosolisation. The ability of the virus to survive PIPAV was assessed in vitro and in vivo by monitoring reporter gene activity. Wistar rats subjected to PIPAV were assessed for any adverse procedure related events. Results: In vitro transduction assays demonstrated that Ad5 retained viability following pressurised aerosolisation and could transduce permissive cells equally effectively as non-aerosolised control vector. PIPAV was well tolerated in rats, although minimal transduction was observed following intraperitoneal administration. Conclusions: PIPAV appears viable and well tolerated, though in vivo efficacy requires further optimisation.
- Subjects :
- peritoneal
aerosolised
medicine.medical_treatment
PIPAC
Pharmaceutical Science
carcinoma
Pharmacology
THERAPY
Article
DELIVERY
03 medical and health sciences
Transduction (genetics)
Pharmacy and materia medica
TYPE-5
0302 clinical medicine
In vivo
oncolytic virus
delivery
peritoneum
PIPAV
peritoneal carcinoma
pressurised
metastasis
Medicine and Health Sciences
medicine
Luciferase
Virotherapy
030304 developmental biology
0303 health sciences
Chemotherapy
Reporter gene
business.industry
CHEMOTHERAPY
CANCER
TUMORS
In vitro
PERITONEAL CARCINOMATOSIS
3. Good health
Oncolytic virus
RS1-441
030220 oncology & carcinogenesis
ANTIBODIES
business
Subjects
Details
- ISSN :
- 19994923
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- Pharmaceutics
- Accession number :
- edsair.doi.dedup.....66f6e6f7734316f9b8688117784e39e3