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Nanostructured paper-based platform for phenylalanine neonatal screening by LED-induced fluorescence
- Source :
- CONICET Digital (CONICET), Consejo Nacional de Investigaciones Científicas y Técnicas, instacron:CONICET
- Publication Year :
- 2020
- Publisher :
- Royal Society of Chemistry, 2020.
-
Abstract
- In this work, a novel paper-based analytical device (PAD) coupled to LED induced fluorescence (LIF) detection (fPAD) for the rapid, selective, and sensitivequantification of phenylalanine (Phe) in neonatal samples was developed. EnzymesPhenylalanine dehydrogenase (PheDH) and diaphorase were immobilized on a papermicrozone previously modified with zinc oxide nanoparticles (ZnONPs) coated withchitosan (CH-ZnONPs). Phe was extracted from the blood spots collected samples on filter paper and was mixed with nicotinamide adenine dinucleotide (NAD+) and resazurin. Then the mixture was deposited on the reaction microzone of the fPAD where PheDH converts the Phe and NAD+ to phenylpyruvate and NADH, respectively.Finally, NADH was oxidized by diaphorase with the consequent reduction fromresazurin to resorufin. This latter was detected by LIF using an excitation wavelength of 535 nm and an emission of 580 nm in a synchronized video microscope. We compare the responses of the PADs with and without nanomaterials to demonstrate the improved analytical performance of the developed devices. For this, the PADs were modified with the same concentration of horseradish peroxidase (HRP). The fluorescent signal obtained from the PADs with nanomaterials was higher than that of the unmodified PADs.Our method exhibited within- and between-assay variation coefficients below 5.23% and 6.67%, was 0.125 μM. The proposed fPAD allowed the simple, rapid, low-cost, and sensitivedetection of Phe in neonatal blood samples. Fil: Moreira, Cristian Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina Fil: Marin Barroso, Evelyn del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina Fil: Pereira, Sirley Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina Fil: Messina, Germán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina Fil: Bertolino, Franco Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
- Subjects :
- FLUOROMETRIC DETECTION
General Chemical Engineering
media_common.quotation_subject
010401 analytical chemistry
PHENYLALANINE
General Engineering
FLUORIMETRIC DETECTION
PAPER-BASED ANALYTICAL DEVICE
ZINC OXIDE 51 NANOPARTICLES
02 engineering and technology
Paper based
Art
ENZYMATIC METHOD
021001 nanoscience & nanotechnology
01 natural sciences
0104 chemical sciences
Analytical Chemistry
purl.org/becyt/ford/1 [https]
purl.org/becyt/ford/1.4 [https]
0210 nano-technology
Humanities
media_common
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- CONICET Digital (CONICET), Consejo Nacional de Investigaciones Científicas y Técnicas, instacron:CONICET
- Accession number :
- edsair.doi.dedup.....66ee79bcf50dbd368888999a108ab903