Back to Search
Start Over
Induction of monocytic differentiation and NF-kappa B-like activities by human immunodeficiency virus 1 infection of myelomonoblastic cells
- Source :
- The Journal of Experimental Medicine
- Publication Year :
- 1992
-
Abstract
- The effects of human immunodeficiency virus 1 (HIV-1) infection on cellular differentiation and NF-kappa B DNA binding activity have been investigated in a new model of myeloid differentiation. PLB-985 cells represent a bipotential myelomonoblastic cell population capable of either granulocytic or monocytic differentiation after induction with appropriate inducers. By virtue of the presence of CD4 on the cell surface, PLB-985 cells were chronically infected with HIV-1 strain IIIB. PLB-IIIB cells clearly possessed a more monocytic phenotype than the parental myeloblasts, as determined by differential staining, increased expression of the myeloid-specific surface markers, and transcription of the c-fms proto-oncogene. NF-kappa B binding activity was inducible by tumor necrosis factor and phorbol myristate acetate in PLB-985. However, in PLB-IIIB cells, constitutive expression of a novel NF-kappa B complex was detected, composed of proteins ranging between 70 and 110 kD. These proteins interacted specifically with the symmetric NF-kappa B site from the interferon beta (IFN-beta) promoter. Mutations affecting the 5' guanine residues of the kappa B site were unable to compete for these NF-kappa B-related proteins. Inducibility of endogenous IFN-beta and IFN-alpha RNA was also increased in PLB-IIIB cells. These studies indicate that HIV-1 infection of myelomonoblastic cells may select for a more mature monocytic phenotype and that unique subunit associations of NF-kappa B DNA binding proteins may contribute to differential NF-kappa B-mediated gene expression.
- Subjects :
- Myeloid
Transcription, Genetic
Cellular differentiation
Immunology
Population
Cell
Molecular Sequence Data
Genes, myc
Gene Expression
Bone Marrow Cells
Biology
DNA-binding protein
Models, Biological
Proto-Oncogene Mas
Monocytes
Bone Marrow
Gene expression
Proto-Oncogenes
medicine
Tumor Cells, Cultured
Immunology and Allergy
Humans
education
education.field_of_study
Acquired Immunodeficiency Syndrome
Base Sequence
Monocyte
NF-kappa B
Interferon-alpha
Cell Differentiation
Genes, fms
Interferon-beta
Articles
Virology
Molecular biology
B vitamins
medicine.anatomical_structure
CD4 Antigens
HIV-1
Virus Activation
Subjects
Details
- ISSN :
- 00221007
- Volume :
- 175
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- The Journal of experimental medicine
- Accession number :
- edsair.doi.dedup.....66ea38f41b29fdfca93c0563bc5322e2