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Dynamic monitoring of CD45-/CD31+/DAPI+ circulating endothelial cells aneuploid for chromosome 8 during neoadjuvant chemotherapy in locally advanced breast cancer
- Source :
- Therapeutic Advances in Medical Oncology, Therapeutic Advances in Medical Oncology, Vol 12 (2020)
- Publication Year :
- 2019
-
Abstract
- Background: Neoadjuvant chemotherapy (NCT) is the standard treatment for patients with locally advanced breast cancer (LABC). The aim of this study was to verify this relationship, and to estimate the clinical value of aneuploid circulating endothelial cells (CECs) in LABC patients with different NCT responses. Methods: Breast cancer patients received an EC4-T4 NCT regimen. Peripheral blood mononuclear cells were obtained before NCT, and after the first and last NCT courses. A novel subtraction enrichment and immunostaining fluorescence in situ hybridization (SE-iFISH) strategy was applied for detection of circulating rare cells (CRCs). CECs (CD45–/CD31+/DAPI+) and circulating tumor cells (CTCs) with different cytogenetic abnormalities related to chromosome 8 aneuploidy were analyzed in LABC patients subjected to NCT. Results: A total of 41 patients were enrolled. Firstly, CD31+/EpCAM+ aneuploid endothelial-epithelial fusion cells were observed in LABC patients. Further, aneuploid CECs in the peripheral blood showed a biphasic response during NCT, as they initially increased and then decreased, whereas a strong positive correlation was observed between aneuploid CECs and CTC numbers. Conclusion: We determined that aneuploid CEC dynamics vary in patients with different response to chemotherapy. Elucidating the potential cross-talk between CTCs and aneuploid CECs may help characterize the process associated with the development of chemotherapy resistance and metastasis.
- Subjects :
- circulating endothelial cells
medicine.medical_treatment
Aneuploidy
circulating tumor cells
lcsh:RC254-282
chemistry.chemical_compound
Circulating tumor cell
Breast cancer
breast cancer
parasitic diseases
medicine
DAPI
aneuploidy
neoadjuvant therapy
Liquid biopsy
Neoadjuvant therapy
Original Research
Chemotherapy
liquid biopsy
business.industry
Standard treatment
medicine.disease
lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Oncology
chemistry
Cancer research
cardiovascular system
business
Subjects
Details
- ISSN :
- 17588340
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Therapeutic advances in medical oncology
- Accession number :
- edsair.doi.dedup.....66e9277928878a4e738266016fd8bf63