Back to Search
Start Over
Polyfunctional CD8+ T-Cell Response to Autologous Peptides from Protease and Reverse Transcriptase of HIV-1 Clade B
- Source :
- Current HIV research. 17(5)
- Publication Year :
- 2019
-
Abstract
- Background: : The diversity of the HIV proteome influences the cellular response and development of an effective vaccine, particularly due to the generation of viral variants with mutations located within CD8+ T-cell epitopes. These mutations can affect the recognition of the epitopes, that may result in the selection of HIV variants with mutated epitopes (autologous epitopes) and different CD8+ T-cell functional profiles. Objective:: To determine the phenotype and functionality of CD8+ T-cell from HIV-infected Colombian patients in response to autologous and consensus peptides derived from HIV-1 clade B protease and reverse transcriptase (RT). Methods:: By flow cytometry, we compared the ex vivo CD8+ T-cell responses from HIV-infected patients to autologous and consensus peptides derived from HIV-1 clade B protease and RT, restricted by HLA-B*35, HLA-B*44 and HLA-B*51 alleles. Results:: Although autologous peptides restricted by HLA-B*35 and HLA-B*44 did not show any differences compared with consensus peptides, we observed the induction of a higher polyfunctional profile of CD8+ T-cells by autologous peptides restricted by HLA-B*51, particularly by the production of interferon-γ and macrophage inflammatory protein-1β. The response by different memory CD8+ T-cell populations was comparable between autologous vs. consensus peptides. In addition, the magnitude of the polyfunctional response induced by the HLA-B*51-restricted QRPLVTIRI autologous epitope correlated with low viremia. Conclusion:: Autologous peptides should be considered for the evaluation of HIV-specific CD8+ Tcell responses and to reveal some relevant epitopes that could be useful for therapeutic strategies aiming to promote polyfunctional CD8+ T-cell responses in a specific population of HIV-infected patients.
- Subjects :
- 0301 basic medicine
Adult
Male
Genotype
HIV Antigens
medicine.medical_treatment
Viremia
HIV Infections
Biology
CD8-Positive T-Lymphocytes
Colombia
Epitope
Cohort Studies
03 medical and health sciences
0302 clinical medicine
Immune system
HIV Protease
Virology
medicine
Cytotoxic T cell
Humans
Immunologic Factors
Protease
Middle Aged
medicine.disease
Flow Cytometry
Reverse transcriptase
HIV Reverse Transcriptase
030104 developmental biology
Infectious Diseases
HIV-1
Female
Ex vivo
CD8
030215 immunology
Subjects
Details
- ISSN :
- 18734251
- Volume :
- 17
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Current HIV research
- Accession number :
- edsair.doi.dedup.....66c4f36e6e441958049b4a04242b638d