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Bridging-induced phase separation induced by cohesin SMC protein complexes
- Source :
- Ryu, J-K, Bouchoux, C, Liu, H W, Kim, E, Minamino, M, de Groot, R, Katan, A J, Bonato, A, Marenduzzo, D, Michieletto, D, Uhlmann, F & Dekker, C 2021, ' Bridging-induced phase separation induced by cohesin SMC protein complexes ', Science Advances, vol. 7, no. 7, eabe5905, pp. 1-10 . https://doi.org/10.1126/sciadv.abe5905, Science Advances 7, Science Advances, Science Advances, 7(7)
- Publication Year :
- 2021
-
Abstract
- Yeast cohesin complexes cluster onto long DNA into liquid droplets, which they accomplish by ATP-independent DNA bridging.<br />Structural maintenance of chromosome (SMC) protein complexes are able to extrude DNA loops. While loop extrusion constitutes a fundamental building block of chromosomes, other factors may be equally important. Here, we show that yeast cohesin exhibits pronounced clustering on DNA, with all the hallmarks of biomolecular condensation. DNA-cohesin clusters exhibit liquid-like behavior, showing fusion of clusters, rapid fluorescence recovery after photobleaching and exchange of cohesin with the environment. Strikingly, the in vitro clustering is DNA length dependent, as cohesin forms clusters only on DNA exceeding 3 kilo–base pairs. We discuss how bridging-induced phase separation, a previously unobserved type of biological condensation, can explain the DNA-cohesin clustering through DNA-cohesin-DNA bridges. We confirm that, in yeast cells in vivo, a fraction of cohesin associates with chromatin in a manner consistent with bridging-induced phase separation. Biomolecular condensation by SMC proteins constitutes a new basic principle by which SMC complexes direct genome organization.
- Subjects :
- Chromosomal Proteins, Non-Histone
Biophysics
Cell Cycle Proteins
Saccharomyces cerevisiae
Chromosomes
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Research Articles
030304 developmental biology
Genomic organization
0303 health sciences
Multidisciplinary
Cohesin
SMC protein
SciAdv r-articles
Life Sciences
Chromosome
Fluorescence recovery after photobleaching
DNA
eye diseases
Yeast
3. Good health
Chromatin
chemistry
biological phenomena, cell phenomena, and immunity
030217 neurology & neurosurgery
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 23752548
- Database :
- OpenAIRE
- Journal :
- Ryu, J-K, Bouchoux, C, Liu, H W, Kim, E, Minamino, M, de Groot, R, Katan, A J, Bonato, A, Marenduzzo, D, Michieletto, D, Uhlmann, F & Dekker, C 2021, ' Bridging-induced phase separation induced by cohesin SMC protein complexes ', Science Advances, vol. 7, no. 7, eabe5905, pp. 1-10 . https://doi.org/10.1126/sciadv.abe5905, Science Advances 7, Science Advances, Science Advances, 7(7)
- Accession number :
- edsair.doi.dedup.....66c381f78a4159ebcc57cd23771649b3