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Correlation between cucumber genotype and resistance to damping-off disease caused by Phytophthora melonis
- Source :
- Biotechnology & Biotechnological Equipment, Vol 33, Iss 1, Pp 1494-1504 (2019)
- Publication Year :
- 2019
- Publisher :
- Informa UK Limited, 2019.
-
Abstract
- Cucumber (Cucumis sativus L.) damping-off disease caused by Phytophthora melonis is a limiting factor affecting cucurbit production. This study assessed a collection of 38 domestic and exotic commercial cucumber genotypes for damping-off resistance at transplanting and 50% flowering. Plants were classified using a four-score scale, the average damping-off percentage ranging from 7.92% (resistant) to 88.01% [highly susceptible (HS)]. Most of the genotypes were susceptible and HS to P. melonis. These genotypes were analyzed with 15 inter-simple sequence repeat (ISSR) markers. A total of 317 bands were produced, of which 297 bands (93.69%) were polymorphic. The primer ISSR29 possessed the highest resolving power, polymorphic information content and marker index value, which could be the most informative primer for distinguishing cucumber genotypes. Cluster analysis and principal component analysis were carried out and there was a probable connection between cucumber genotypes and resistance level against P. melonis, indicating possible application for ISSR in studying disease resistance of cucumber and producing cultivars that resist damping-off. The expression of three candidate genes probably involved in the defence against P. melonis was quantified by quantitative real-time polymerase chain reaction in five cucumber genotypes showing different susceptibility to the pathogen. CsWRKY20, CsLecRK6.1 and LOX1 genes were differentially expressed depending on genotype in early stages of infection. LOX1 was the most expressed gene across experiments and the one that best discriminated between susceptible and resistant genotypes. These results are a valuable resource for future functional genomics studies to unravel the molecular mechanisms of C. sativus/P. melonis interaction.
- Subjects :
- 0106 biological sciences
Limiting factor
lcsh:Biotechnology
Damping off
Disease
01 natural sciences
03 medical and health sciences
phytophthora melonis
lcsh:TP248.13-248.65
Genetic variation
Genotype
Phytophthora melonis
030304 developmental biology
0303 health sciences
biology
Resistance (ecology)
issr markers
food and beverages
biology.organism_classification
Horticulture
cucumis sativus
genetic variation
gene expression
Cucumis
010606 plant biology & botany
Biotechnology
Subjects
Details
- ISSN :
- 13143530 and 13102818
- Volume :
- 33
- Database :
- OpenAIRE
- Journal :
- Biotechnology & Biotechnological Equipment
- Accession number :
- edsair.doi.dedup.....66b9122d03929c077dcbbaa24b5f1fd2