Effect of Testosterone on the Biosynthesis of Phosphatidylglycerol from L-α:-Glycerophosphate-2-3H by Whole Homogenate and Mitochondria Isolated from Rat Ventral Prostate

The formation of labeled phosphatidylglycerol from L-α-glycerophosphate-2-3H and CDP-D-diglyceride has been demonstrated in homogenate and mitochondria isolated from rat ventral prostate. In addition, labeled phosphatidylglycerophosphate was detected as a minor biosynthesized lipid. Homogenates and mitochondria isolated from prostates after castration of rats have a decreased ability to incorporate L-α-glycerophosphate-2-3H and CDP-D-diglyceride into phospholipids, but this ability is restored after the administration of testosterone to the castrated animals. The composition of synthesized phospholipids by homogenates and mitochondria isolated from normal and sham-operated rats, and from normal and castrated rats treated with testosterone, is very similar; the major labeled lipid (ca. 90%) being phosphatidylglycerol. This composition is very different when mitochondria isolated from prostates of castrated rats were incubated with the same substrates: labeled phosphatidylglycerol (ca. 55-58%) and phosphati...