Back to Search
Start Over
SOX17 promoter methylation in plasma circulating tumor DNA of patients with non-small cell lung cancer
- Source :
- Clinical Chemistry and Laboratory Medicine (CCLM). 54
- Publication Year :
- 2016
- Publisher :
- Walter de Gruyter GmbH, 2016.
-
Abstract
- Background SOX17 belongs to the high-mobility group-box transcription factor superfamily and down-regulates the Wnt pathway. The aim of our study was to evaluate the prognostic significance of SOX17 promoter methylation in circulating tumor DNA (ctDNA) in plasma of non-small cell lung cancer (NSCLC) patients. Methods We examined the methylation status of SOX17 promoter in 57 operable NSCLC primary tumors and paired adjacent non-cancerous tissues and in ctDNA isolated from 48 corresponding plasma samples as well as in plasma from 74 patients with advanced NSCLC and 49 healthy individuals. SOX17 promoter methylation was examined by Methylation Specific PCR (MSP). Results In operable NSCLC, SOX17 promoter was fully methylated in primary tumors (57/57, 100%), and in corresponding ctDNA (27/48, 56.2%) while it was detected in only 1/49 (2.0%) healthy individuals. In advanced NSCLC, SOX17 promoter was methylated in ctDNA in 27/74 (36.4%) patients and OS was significantly different in favor of patients with non-methylated SOX17 promoter (p=0.012). Multivariate analysis revealed that SOX17 promoter methylation in ctDNA was an independent prognostic factor associated with OS in patients with advanced but not operable NSCLC. Conclusions Our results show that SOX17 promoter is highly methylated in primary tumors and in corresponding plasma samples both in operable and advanced NSCLC. In the advanced setting, SOX17 promoter methylation in plasma ctDNA has a statistical significant influence on NSCLC patient's survival time. Detection of SOX17 promoter methylation in plasma provides prognostic information and merits to be further evaluated as a circulating tumor biomarker in patients with operable and advanced NSCLC.
- Subjects :
- Male
0301 basic medicine
Pathology
medicine.medical_specialty
Lung Neoplasms
animal structures
Clinical Biochemistry
Bisulfite sequencing
Biology
03 medical and health sciences
0302 clinical medicine
Carcinoma, Non-Small-Cell Lung
SOXF Transcription Factors
Carcinoma
medicine
Humans
Promoter Regions, Genetic
Lung cancer
Transcription factor
Aged
Biochemistry (medical)
DNA, Neoplasm
General Medicine
Methylation
DNA Methylation
medicine.disease
respiratory tract diseases
030104 developmental biology
Cell-free fetal DNA
030220 oncology & carcinogenesis
embryonic structures
DNA methylation
Cancer research
Biomarker (medicine)
Female
Subjects
Details
- ISSN :
- 14374331 and 14346621
- Volume :
- 54
- Database :
- OpenAIRE
- Journal :
- Clinical Chemistry and Laboratory Medicine (CCLM)
- Accession number :
- edsair.doi.dedup.....66b36dcda1250ed0d8c491870d9860d4