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LHRH might act as a negative autocrine regulator of proliferation of human ovarian cancer
- Source :
- European Journal of Endocrinology. :665-670
- Publication Year :
- 2000
- Publisher :
- Oxford University Press (OUP), 2000.
-
Abstract
- OBJECTIVE: More than 80% of human ovarian cancers express LHRH and its receptor. The proliferation of human ovarian cancer cell lines is reduced by both LHRH agonists and antagonists. This study was designed to further clarify the possible biological function of this LHRH system. DESIGN: As LHRH agonists and antagonists uniformly reduce proliferation of human ovarian cancer in a dose-dependent way, the effect of low concentrations of authentic LHRH was studied. In addition, longer periods of treatment (up to 9 days) were analyzed. To assess the physiological role of LHRH produced by ovarian cancer cells it was neutralized by adequate concentrations of a specific LHRH antiserum. METHODS: Human ovarian cancer cells EFO-21 and EFO-27, which express LHRH and its receptor, were incubated for 1-9 days with increasing concentrations (1pmol/l to 10 micromol/l) of authentic LHRH or with concentrations of LHRH antiserum capable of neutralizing at least 1nmol/l LHRH. Proliferation was assessed by counting cells. RESULTS AND CONCLUSIONS: Authentic LHRH reduced time- and dose-dependently proliferation (by maximally mean+/-s.e.m. 32.7 +/- 4.4%, Newman-Keuls, P < 0.001) of both ovarian cancer cell lines. At very low concentrations (1pmol/l) a marginal reduction of proliferation or no effect was observed. A mitogenic effect of authentic LHRH was never detected. Treatment of ovarian cancer cell cultures with antiserum to LHRH significantly increased (up to mean+/-s.e.m. 121.0 +/- 2.8% of controls, Newman-Keuls P
- Subjects :
- endocrine system
medicine.medical_specialty
Time Factors
Endocrinology, Diabetes and Metabolism
Ovary
Biology
Cell Line
Gonadotropin-Releasing Hormone
03 medical and health sciences
0302 clinical medicine
Endocrinology
Internal medicine
medicine
Humans
Receptor
Autocrine signalling
030304 developmental biology
Ovarian Neoplasms
0303 health sciences
Dose-Response Relationship, Drug
Cell growth
Immune Sera
General Medicine
medicine.disease
Adenocarcinoma, Mucinous
In vitro
Cystadenocarcinoma, Serous
3. Good health
Adenocarcinoma, Papillary
Autocrine Communication
medicine.anatomical_structure
Tumor progression
Cell culture
030220 oncology & carcinogenesis
Female
Ovarian cancer
Cell Division
hormones, hormone substitutes, and hormone antagonists
Subjects
Details
- ISSN :
- 1479683X and 08044643
- Database :
- OpenAIRE
- Journal :
- European Journal of Endocrinology
- Accession number :
- edsair.doi.dedup.....668df111ffa019aa1f17a5c9cd28d2e8