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Simplified quantification method for in vivo SPECT/CT imaging of asialoglycoprotein receptor with 99mTc-p(VLA-co-VNI) to assess and stage hepatic fibrosis in mice
- Source :
- Scientific Reports
- Publication Year :
- 2016
- Publisher :
- Nature Publishing Group, 2016.
-
Abstract
- The goal of this study is to develop a noninvasive method of SPECT imaging to quantify and stage liver fibrosis with an Asialoglycoprotein receptor (ASGP-R) targeting tracer—99mTc-p(VLA-co-VNI). ASGP-Rs are well known to specifically express in the mammalian liver. Here, we demonstrated ASGP-R expression decreased in carbon tetrachloride (CCl4)-induced mouse model. ASGP-R expression correlated with liver fibrosis progression. ASGP-R could be a useful marker in the stage of liver fibrosis. Liver uptake value (LUV) derived by SPECT imaging was used to assess liver fibrosis in the CCl4-induced mouse model. LUV = [radioactivity (liver uptake)/radioactivity (injected)] × 100/liver volume. The LUV decreased along with the disease progression. The relationships between LUV and liver hydroxyproline (i.e. collagen), as well as Sirius Red were established and verified. A strong negative linear correlation was found between LUV and hydroxyproline levels (r = −0.83) as well as LUV and Sirius Red quantification (r = −0.83). In conclusion, SPECT imaging with 99mTc-p(VLA-co-VNI) is useful in evaluating and staging liver fibrosis in vivo.
- Subjects :
- Liver Cirrhosis
Pathology
medicine.medical_specialty
Single Photon Emission Computed Tomography Computed Tomography
Polymers
Asialoglycoprotein Receptor
Article
030218 nuclear medicine & medical imaging
03 medical and health sciences
Hydroxyproline
chemistry.chemical_compound
Mice
0302 clinical medicine
In vivo
Spect imaging
medicine
Animals
Sirius Red
Multidisciplinary
medicine.diagnostic_test
business.industry
Technetium
chemistry
030220 oncology & carcinogenesis
Carbon tetrachloride
Asialoglycoprotein receptor
Liver function tests
business
Hepatic fibrosis
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....668abc893ccaef5f244b2cb6db800150
- Full Text :
- https://doi.org/10.1038/srep25377