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Impedimetric immunosensor based on gold nanoparticles modified graphene paper for label-free detection of Escherichia coli O157:H7
- Source :
- Biosensorsbioelectronics. 49
- Publication Year :
- 2013
-
Abstract
- In this study, a low-cost and robust impedimetric immunosensor based on gold nanoparticles modified free-standing graphene paper electrode for rapid and sensitive detection of Escherichia coli O157:H7 (E. coli O157:H7) was developed. Graphene paper was prepared by chemical reduction of graphene oxide paper obtained from vacuum filtration method. Scanning electron microscope, Raman spectroscopy and X-ray diffraction techniques were employed to investigate the surface morphology and crystal structure of the prepared graphene paper. The gold nanoparticles were grown on the surface of graphene paper electrode by one-step electrodeposition technique. The immobilization of anti-E. coli O157:H7 antibodies on paper electrode were performed via biotin-streptavidin system. Electrochemical impedance spectroscopy was used to detect E. coli O157:H7 captured on the paper electrode. Results show that the developed paper immunosensor possesses greatly enhanced sensing performance, such as wide linear range (1.5 × 10(2)-1.5 × 10(7) cfu mL(-1)), low detection limit (1.5 × 10(2) cfu mL(-1)), and excellent specificity. Furthermore, flexible test demonstrate the graphene paper based sensing device has high tolerability to mechanical stress. The strategy of structurally integrating metal nanomaterials, graphene paper, and biorecognition molecules would provide new insight into design of flexible immunosensors for routine sensing applications.
- Subjects :
- Paper
Materials science
Biomedical Engineering
Biophysics
Nanotechnology
Biosensing Techniques
Escherichia coli O157
Nanomaterials
symbols.namesake
Limit of Detection
Electrochemistry
Humans
Escherichia coli Infections
Graphene oxide paper
Detection limit
Immunoassay
General Medicine
Dielectric spectroscopy
Linear range
Colloidal gold
Dielectric Spectroscopy
Electrode
symbols
Nanoparticles
Graphite
Gold
Raman spectroscopy
Antibodies, Immobilized
Biotechnology
Subjects
Details
- ISSN :
- 18734235
- Volume :
- 49
- Database :
- OpenAIRE
- Journal :
- Biosensorsbioelectronics
- Accession number :
- edsair.doi.dedup.....6689f92cc5d440854af554a39dd1a6d8