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Probiotic-Derived Polyphosphate Accelerates Intestinal Epithelia Wound Healing through Inducing Platelet-Derived Mediators
- Source :
- Mediators of Inflammation, Mediators of Inflammation, Vol 2021 (2021)
- Publication Year :
- 2021
- Publisher :
- Hindawi, 2021.
-
Abstract
- Inflammatory bowel disease (IBD), such as ulcerative colitis (UC) and Crohn’s disease (CD), is an intractable intestinal inflammation associated with the disruption of the intestinal mucosa. We previously demonstrated that Lactobacillus brevis-derived long-chain polyphosphate (poly P) improved the intestinal barrier function by the upregulation of cell adhesion and relieved intestinal inflammation, thereby exerting a curing effect on colitis in vitro, in vivo, and in an investigator-initiated clinical study of UC. However, how poly P improves mucosal defects induced by intestinal inflammation has not been elucidated. In this study, we detected the accumulation of platelets in inflamed tissues induced by poly P in a dextran sulfate sodium- (DSS-) induced colitis mouse model. A light transmission aggregometry analysis and scanning electron microscopy showed that poly P promoted the platelet aggregation. An SRB assay and ki-67 staining showed that the supernatant of poly P-treated platelet-rich plasma (PRP) increased intestinal epithelial cell growth. A wound healing assay showed that the supernatant of poly P-treated PRP, but not poly P itself, accelerated wound healing. A Western blotting analysis indicated that mitogen-activated protein kinase activation was induced by the supernatant of poly P-treated human PRP in the epithelial cells and its wound healing effect was significantly decreased by the inhibition of ERK signaling. These data suggested that platelet-derived mediators induced by poly P improved intestinal inflammation through the promotion of epithelial cell growth by the activation of the ERK signaling pathway. The mechanism is a novel host-microbe interaction through mammalian platelet-derived mediators induced by bacterial molecules.
- Subjects :
- 0301 basic medicine
Platelet Aggregation
Article Subject
Immunology
Inflammatory bowel disease
Mice
03 medical and health sciences
0302 clinical medicine
Downregulation and upregulation
Intestinal mucosa
Polyphosphates
In vivo
Pathology
medicine
RB1-214
Animals
Intestinal Mucosa
Colitis
Extracellular Signal-Regulated MAP Kinases
Cell adhesion
Cells, Cultured
Barrier function
Mice, Inbred BALB C
Wound Healing
Chemistry
Probiotics
Cell Biology
Platelet Activation
medicine.disease
Molecular biology
030104 developmental biology
030211 gastroenterology & hepatology
Wound healing
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 09629351
- Database :
- OpenAIRE
- Journal :
- Mediators of Inflammation
- Accession number :
- edsair.doi.dedup.....6689be47e5c36b6b94f61f1415e7cbc8
- Full Text :
- https://doi.org/10.1155/2021/5582943