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Microtubule Network Facilitates Nuclear Targeting of Human Cytomegalovirus Capsid
- Source :
- Journal of Virology. 77:8541-8547
- Publication Year :
- 2003
- Publisher :
- American Society for Microbiology, 2003.
-
Abstract
- We assessed the requirement of the host cytoskeleton for the intracytosolic transport of incoming human cytomegalovirus (HCMV) capsids. Treatments with microtubule (MT)-depolymerizing drugs nocodazole and colchicine led to a drastic decrease in levels of IE1 antigen, whereas cytochalasin B had no effect on the level of IE1 as determined by Western blot analyses. Sequential treatment including nocodazole washout and removal of cell surface virion revealed that HCMV entry into the cells occurred normally in the absence of the MT network. This finding was also supported by data obtained by monitoring pUL83 signals with an immunofluorescent assay (IFA). Furthermore, we demonstrated a close association of incoming HCMV capsids with MTs by IFA and ultrastructural analyses. In the absence of the MT network, the capsids which had entered the cytoplasm did not move to close proximity of the nucleus. These data suggest that HCMV capsids associate with the MT network to facilitate their own movement to the nucleus before the onset of immediate-early (IE) gene expression and that this association is required to start efficient IE gene expression.
- Subjects :
- Gene Expression Regulation, Viral
Human cytomegalovirus
Cytoplasm
Cytochalasin B
viruses
Immunology
Cytomegalovirus
Fluorescent Antibody Technique
Biology
Microtubules
Microbiology
Immediate early protein
Cell Line
Immediate-Early Proteins
Viral Proteins
chemistry.chemical_compound
Capsid
Microtubule
Virology
medicine
Humans
Cell Nucleus
Nocodazole
virus diseases
Biological Transport
biochemical phenomena, metabolism, and nutrition
medicine.disease
Molecular biology
Virus-Cell Interactions
Microscopy, Electron
Cell nucleus
medicine.anatomical_structure
chemistry
Insect Science
Caco-2 Cells
Host cytoskeleton
Colchicine
Subjects
Details
- ISSN :
- 10985514 and 0022538X
- Volume :
- 77
- Database :
- OpenAIRE
- Journal :
- Journal of Virology
- Accession number :
- edsair.doi.dedup.....6680eba764261781680f13551e777715