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Impact of manganese on the hippocampus metabolism in the context of MEMRI: a proton HRMAS MRS study
- Source :
- Toxicology Research, Toxicology Research, Royal Society of Chemistry, 2015, 4 (2), pp.376-384. ⟨10.1039/c4tx00135d⟩
- Publication Year :
- 2015
- Publisher :
- HAL CCSD, 2015.
-
Abstract
- Manganese enhanced MRI (MEMRI) offers many possibilities such as tract tracing of neuronal pathways and functional imaging in vivo. This technique necessitates a direct or indirect acute injection of MnCl2 in the brain. Unfortunately, local concentrations of Mn2+ and its impact on metabolism after a single injection remain largely unknown. In this study, we combined in vivo MEMRI and ex vivo Proton High Resolution Magic Angle Spinning MRS (1H HRMAS MRS) to investigate the delayed impact of Mn2+ on rat hippocampal metabolism. MEMRI images were acquired 24 h after MnCl2 injection in the dentate gyrus of the rat hippocampus at two different Mn2+ doses: low (8 nmol; n = 16) and high (500 nmol; n = 10). The low Mn2+ dose had almost no impact on hippocampal metabolism while the high dose altered the amplitude of several metabolites (up to +54% for Glu and −71% for Asp). Moreover, at this high dose, the paramagnetic properties of Mn2+ led to a broadening of the resonances of several organic acids (lactate, glutamate, N-acetyl aspartate etc.), suggesting a chelation of Mn2+ and an impact on Mn2+ relaxivity. Metabolite amplitudes were well correlated with the Mn2+ concentrations measured with an MRI T1-map (glutamate: R2 = 0.8, p = 0.02; phosphoethanolamine: R2 = 0.9, p = 0.0004; γ-aminobutyrate: R2 = 0.7, p = 0.005 and phosphocholine: R2 = 0.6, p = 0.04). To conclude, HRMAS is well suited to investigate Mn2+ impact on the metabolism. The low Mn2+ dose (8 nmol) usually used in the MEMRI experiment does not impact the hippocampal metabolism. The chelation of Mn2+ and its impact on relaxivity suggests an over-estimation of the Mn2+ concentration when measured through a T1 map.
- Subjects :
- inorganic chemicals
Chemistry
Health, Toxicology and Mutagenesis
Metabolite
Dentate gyrus
Glutamate receptor
Hippocampus
Context (language use)
Hippocampal formation
Toxicology
030218 nuclear medicine & medical imaging
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Nuclear magnetic resonance
In vivo
[SDV.TOX]Life Sciences [q-bio]/Toxicology
030217 neurology & neurosurgery
ComputingMilieux_MISCELLANEOUS
Phosphocholine
Subjects
Details
- Language :
- English
- ISSN :
- 2045452X and 20454538
- Database :
- OpenAIRE
- Journal :
- Toxicology Research, Toxicology Research, Royal Society of Chemistry, 2015, 4 (2), pp.376-384. ⟨10.1039/c4tx00135d⟩
- Accession number :
- edsair.doi.dedup.....667fe349beeda8f6e845ef65c8fbab40