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Expression and functional analysis of putative vacuolar Ca2+-transporters (CAXs and ACAs) in roots of salt tolerant and sensitive rice cultivars
- Source :
- Protoplasma. 251:1067-1075
- Publication Year :
- 2014
- Publisher :
- Springer Science and Business Media LLC, 2014.
-
Abstract
- Vacuolar Ca2+-transporters could play an important role for salt tolerance in rice (Oryza sativa L.) root. Here, we compared the expression profiles of putative vacuolar cation/H+ exchanger (CAX) and calmodulin-regulated autoinhibited Ca2+-ATPase (ACA) in rice roots of salt tolerant cv. Pokkali and salt sensitive cv. IR29. In addition to five putative vacuolar CAX genes in the rice genome, a new CAX gene (OsCAX4) has been annotated. In the present study, we isolated the OsCAX4 gene and showed that its encoded protein possesses a unique transmembrane structure and is potentially involved in transporting not only Ca2+ but also Mn2+ and Cu2+. These six OsCAX genes differed in their mRNA expression pattern in roots of tolerant versus sensitive rice cultivars exposed to salt stress. For example, OsCAX4 showed abundant expression in IR29 (sensitive) upon prolonged salt stress. The mRNA expression profile of four putative vacuolar Ca2+-ATPases (OsACA4-7) was also examined. Under control conditions, the mRNA levels of OsACA4, OsACA5, and OsACA7 were relatively high and similar among IR29 and Pokkali. Upon salt stress, only OsACA4 showed first a decrease in its expression in Pokkali (tolerant), followed by a significant increase. Based on these results, a role of vacuolar Ca2+ transporter for salt tolerance in rice root was discussed.
- Subjects :
- Sequence analysis
Molecular Sequence Data
Calcium-Transporting ATPases
Plant Science
Vacuole
Sodium Chloride
Biology
Plant Roots
Antiporters
Gene Expression Regulation, Plant
Botany
Magnesium
Amino Acid Sequence
RNA, Messenger
Cloning, Molecular
Cation Transport Proteins
Gene
chemistry.chemical_classification
Regulation of gene expression
Oryza sativa
Base Sequence
Sequence Homology, Amino Acid
food and beverages
RNA
Biological Transport
Oryza
Salt-Tolerant Plants
Transporter
Sequence Analysis, DNA
Cell Biology
General Medicine
Amino acid
Biochemistry
chemistry
Calcium
Sequence Alignment
Copper
Subjects
Details
- ISSN :
- 16156102 and 0033183X
- Volume :
- 251
- Database :
- OpenAIRE
- Journal :
- Protoplasma
- Accession number :
- edsair.doi.dedup.....667c8c636bbb232815119abe8ac70109