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Complex target SELEX-based identification of DNA aptamers against Bungarus caeruleus venom for the detection of envenomation using a paper-based device
- Source :
- Biosensorsbioelectronics. 193
- Publication Year :
- 2021
-
Abstract
- Complex target SELEX always have been an intriguing approach to the scientific community, as it offers the potential discovery of novel biomarkers. We herein successfully performed SELEX on Bungarus caeruleus venom to develop a panel of highly affine aptamers that specifically recognizes the B. caeruleus (common krait) venom and was able to discriminate the B. caeruleus venom from Cobra, Russell's, and Saw-scaled viper's venom. The aptamers generated against the crude venom also lead to the identification of the specific component of the venom, which is β-Bungarotoxin, a toxin uniquely present in the B. caeruleus venom. The best performing aptamer candidates were used as a molecular recognition element in a paper-based device and were able to detect as low as 2 ng krait venom in human serum background. The developed aptamer-based paper device can be used for potential point-of-care venom detection applications due to its simplicity and affordability.
- Subjects :
- Elapid Venoms
Bungarus
biology
Aptamer
Biomedical Engineering
Biophysics
Venom
Cobra
General Medicine
Computational biology
Biosensing Techniques
Aptamers, Nucleotide
biology.organism_classification
Bungarotoxins
Snake venom
Electrochemistry
Animals
Humans
Common krait
Envenomation
computer
Systematic evolution of ligands by exponential enrichment
Biotechnology
computer.programming_language
Subjects
Details
- ISSN :
- 18734235
- Volume :
- 193
- Database :
- OpenAIRE
- Journal :
- Biosensorsbioelectronics
- Accession number :
- edsair.doi.dedup.....6673e8f13a32c83435da22885a26376e