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Near‐infrared photoimmunotherapy (NIR‐PIT) on cholangiocarcinoma using a novel catheter device with light emitting diodes
- Source :
- Cancer Science
- Publication Year :
- 2021
- Publisher :
- Wiley, 2021.
-
Abstract
- Near‐infrared photoimmunotherapy (NIR‐PIT) is a novel therapy for cancers that uses NIR light and antibody‐photosensitizer (IR700) conjugates. However, it is difficult to deliver NIR light into the bile duct for cholangiocarcinoma (CCA) from the conventional extracorporeal apparatus. Thus, in this study, we developed a dedicated catheter with light emitting diodes (LEDs) that supersedes conventional external irradiation devices; we investigated the therapeutic effect of NIR‐PIT for CCA using the novel catheter. The new catheter was designed to be placed in the bile duct and a temperature sensor was attached to the tip of the catheter to avoid thermal burn. An anti‐epidermal growth factor receptor (EGFR) antibody, Panitumumab‐IR700 conjugate or anti‐human epidermal growth factor receptor type 2 (HER2) antibody, Trastuzumab‐IR700 conjugate, was used with EGFR‐ or HER2‐expressing cell lines, respectively. The in vitro efficacy of NIR‐PIT was confirmed in cultured cells; the capability of the new catheter for NIR‐PIT was then tested in a mouse tumor model. NIR‐PIT via the developed catheter treated CCA xenografts in mice. NIR‐PIT had an effect in Panitumumab‐IR700 conjugate‐ and Trastuzumab‐IR700 conjugate‐treated CCA cells that depended on the receptor expression level. Tumor growth was significantly suppressed in mice treated with NIR‐PIT using the novel catheter compared with controls (P<br />A new catheter with light emitting diodes for photoimmunotherapy for cholangiocarcinoma was developed. The new catheter was designed to be placed in the bile duct and a temperature sensor was attached to the tip of the catheter to avoid thermal burn. Tumor growth was significantly suppressed in mice treated with NIR‐PIT using the novel catheter.
- Subjects :
- 0301 basic medicine
Cancer Research
near‐infrared photoimmunotherapy
near-infrared photoimmunotherapy
Receptor expression
law.invention
Mice
0302 clinical medicine
law
Epidermal growth factor receptor
Mice, Inbred BALB C
Photosensitizing Agents
biology
Bile duct
Chemistry
Panitumumab
catheter
General Medicine
light emitting diode
Catheter
surgical procedures, operative
medicine.anatomical_structure
Oncology
030220 oncology & carcinogenesis
Female
Original Article
Immunotherapy
cholangiocarcinoma
Light-emitting diode
Catheters
Infrared Rays
Mice, Nude
03 medical and health sciences
Growth factor receptor
Cell Line, Tumor
medicine
Animals
Humans
Low-Level Light Therapy
neoplasms
technology, industry, and agriculture
Photoimmunotherapy
Original Articles
Trastuzumab
equipment and supplies
Xenograft Model Antitumor Assays
Drug Discovery and Delivery
030104 developmental biology
Bile Duct Neoplasms
biology.protein
Biomedical engineering
Conjugate
Subjects
Details
- ISSN :
- 13497006 and 13479032
- Volume :
- 112
- Database :
- OpenAIRE
- Journal :
- Cancer Science
- Accession number :
- edsair.doi.dedup.....666d611b0deec2b4714d50f030a2b9ae