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Alpha 2-macroglobulin-proteinase complexes, plasminogen activator inhibitor type-1-plasminogen activator complexes, and receptor-associated protein bind to a region of the alpha 2-macroglobulin receptor containing a cluster of eight complement-type repeats
- Source :
- Moestrup, S K, Holtet, T L, Etzerodt, M, Thøgersen, H C, Nykjær, A, Andreasen, P, Rasmussen, H H, Sottrup-Jensen, L & Gliemann, J 1993, ' Alpha 2-macroglobulin-proteinase complexes, plasminogen activator inhibitor type-1-plasminogen activator complexes, and receptor-associated protein bind to a region of the alpha(2)-Macroglobulin receptor containing a cluster of eight complement-type repeats ', Journal of Biological Chemistry, vol. 268, no. 18, pp. 13691-13696 .
- Publication Year :
- 1993
- Publisher :
- Elsevier BV, 1993.
-
Abstract
- A region containing sites for ligand binding was localized in the 4525-amino acid residue alpha 2-macroglobulin receptor/low density lipoprotein receptor-related protein (alpha 2MR/LRP) by ligand- and immunoblotting of proteinase and CNBr digests of the purified human placental protein. 125I-Labeled rat alpha 1-macroglobulin light chain, urokinase-plasminogen activator inhibitor type-1 complex, and alpha 2MR-associated protein all bound to a 75-kDa CNBr-generated fragment (68 kDa after deglycosylation). In addition to the three ligands, the fragment bound a novel monoclonal antibody reacting in the region defined by amino acid residues 1165-1246 as determined by binding to recombinant fragments of alpha 2MR/LRP. The positions of methionine residues in alpha 2MR/LRP suggested that the ligand-binding CNBr fragment contained three disulfide-linked peptides comprising the residues 776-1399. This origin was confirmed by partial amino acid sequencing of the electroblotted fragment and polypeptides generated by reduction of the fragment. The identified region represents 13.6% of the molecular mass (nonglycosylated) of alpha 2MR/LRP and contains one of three large clusters of complement-type repeats.
- Subjects :
- chemistry.chemical_classification
biology
Activator (genetics)
Cell Biology
Ligand (biochemistry)
Biochemistry
Molecular biology
Amino acid
LDL-receptor-related protein-associated protein
alpha-2-Macroglobulin
chemistry
biology.protein
Binding site
Molecular Biology
Peptide sequence
Plasminogen activator
Subjects
Details
- ISSN :
- 00219258
- Volume :
- 268
- Database :
- OpenAIRE
- Journal :
- Journal of Biological Chemistry
- Accession number :
- edsair.doi.dedup.....66476989b8e598e750ed781b30f602cd