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Alternative splicing in the Caenorhabditis elegans DNA topoisomerase I gene
- Source :
- Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression. 1396:207-214
- Publication Year :
- 1998
- Publisher :
- Elsevier BV, 1998.
-
Abstract
- 5′-end cDNA fragments of the Caenorhabditis elegans DNA topoisomerase I gene were obtained by rapid amplification of the cDNA ends from C. elegans mRNAs. The presence of a SL1 sequence at the 5′-terminus of the cDNA sequence suggested trans -splicing of the pre-mRNA. By comparing the complete cDNA sequence with the genomic λ DNA clones, the gene structure composed of five exons was established. Alternative splicing deleting the second exon was observed in the cDNA fragments obtained by a gene-specific reverse transcription followed by polymerase chain reactions. The shorter mRNA missing the second exon was expressed at all the developmental stages, while the full-length mRNA was present only in embryos.
- Subjects :
- DNA, Complementary
Base Sequence
cDNA library
Molecular Sequence Data
Alternative splicing
Biophysics
RNA-Directed DNA Polymerase
Biology
Polymerase Chain Reaction
Biochemistry
Molecular biology
Alternative Splicing
Exon
Exon trapping
DNA Topoisomerases, Type I
Rapid amplification of cDNA ends
Structural Biology
Complementary DNA
RNA splicing
RNA Precursors
Genetics
Animals
Amino Acid Sequence
Caenorhabditis elegans
Gene
Subjects
Details
- ISSN :
- 01674781
- Volume :
- 1396
- Database :
- OpenAIRE
- Journal :
- Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression
- Accession number :
- edsair.doi.dedup.....6640044b38e2e96b34cccabfb8dc958d