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Promoting microbial utilization of phenolic substrates from bio-oil
- Source :
- Journal of Industrial Microbiology and Biotechnology. 46:1531-1545
- Publication Year :
- 2019
- Publisher :
- Oxford University Press (OUP), 2019.
-
Abstract
- The economic viability of the biorefinery concept is limited by the valorization of lignin. One possible method of lignin valorization is biological upgrading with aromatic-catabolic microbes. In conjunction, lignin monomers can be produced by fast pyrolysis and fractionation. However, biological upgrading of these lignin monomers is limited by low water solubility. Here, we address the problem of low water solubility with an emulsifier blend containing approximately 70 wt% Tween® 20 and 30 wt% Span® 80. Pseudomonas putida KT2440 grew to an optical density (OD600) of 1.0 ± 0.2 when supplied with 1.6 wt% emulsified phenolic monomer-rich product produced by fast pyrolysis of red oak using an emulsifier dose of 0.076 ± 0.002 g emulsifier blend per g of phenolic monomer-rich product. This approach partially mitigated the toxicity of the model phenolic monomer p-coumarate to the microbe, but not benzoate or vanillin. This study provides a proof of concept that processing of biomass-derived phenolics to increase aqueous availability can enhance microbial utilization.
- Subjects :
- 0301 basic medicine
Bioengineering
Fractionation
Chemical Fractionation
Lignin
01 natural sciences
Applied Microbiology and Biotechnology
03 medical and health sciences
chemistry.chemical_compound
Phenols
Bioenergy
Plant Oils
Biomass
Food science
Pseudomonas putida
010405 organic chemistry
Chemistry
Vanillin
Polyphenols
Biorefinery
0104 chemical sciences
030104 developmental biology
Emulsion
Emulsions
Pyrolysis
Biotechnology
Subjects
Details
- ISSN :
- 14765535 and 13675435
- Volume :
- 46
- Database :
- OpenAIRE
- Journal :
- Journal of Industrial Microbiology and Biotechnology
- Accession number :
- edsair.doi.dedup.....6635947626fe3adf92f84b6a5479a21b