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Human organoid biofilm model for assessing antibiofilm activity of novel agents
- Source :
- NPJ Biofilms and Microbiomes, npj Biofilms and Microbiomes, Vol 7, Iss 1, Pp 1-13 (2021), npj Biofilms and Microbiomes, 7(1). NATURE RESEARCH
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- Bacterial biofilms cause 65% of all human infections and are highly resistant to antibiotic therapy but lack specific treatments. To provide a human organoid model for studying host-microbe interplay and enabling screening for novel antibiofilm agents, a human epidermis organoid model with robust methicillin-resistant Staphylococcus aureus (MRSA) USA300 and Pseudomonas aeruginosa PAO1 biofilm was developed. Treatment of 1-day and 3-day MRSA and PAO1 biofilms with antibiofilm peptide DJK-5 significantly and substantially reduced the bacterial burden. This model enabled the screening of synthetic host defense peptides, revealing their superior antibiofilm activity against MRSA compared to the antibiotic mupirocin. The model was extended to evaluate thermally wounded skin infected with MRSA biofilms resulting in increased bacterial load, cytotoxicity, and pro-inflammatory cytokine levels that were all reduced upon treatment with DJK-5. Combination treatment of DJK-5 with an anti-inflammatory peptide, 1002, further reduced cytotoxicity and skin inflammation.
- Subjects :
- Methicillin-Resistant Staphylococcus aureus
0301 basic medicine
medicine.drug_class
030106 microbiology
Antibiotics
Anti-Inflammatory Agents
Drug Evaluation, Preclinical
Mupirocin
medicine.disease_cause
Models, Biological
Applied Microbiology and Biotechnology
Microbiology
Article
Microbial ecology
03 medical and health sciences
chemistry.chemical_compound
medicine
Organoid
Humans
Cytotoxicity
Skin
Epidermis (botany)
Antimicrobials
Pseudomonas aeruginosa
Chemistry
QR100-130
Biofilm
biochemical phenomena, metabolism, and nutrition
bacterial infections and mycoses
Bacterial Load
Anti-Bacterial Agents
Organoids
030104 developmental biology
Staphylococcus aureus
Biofilms
Drug Therapy, Combination
Burns
Oligopeptides
Biotechnology
Subjects
Details
- ISSN :
- 20555008
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- npj Biofilms and Microbiomes
- Accession number :
- edsair.doi.dedup.....6605a983c257efdf568a3ce7a32c1181