Back to Search
Start Over
Involvement of abscisic acid, ABI5, and PPC2 in plant acclimation to low CO2
- Source :
- Journal of Experimental Botany
- Publication Year :
- 2020
- Publisher :
- Oxford University Press (OUP), 2020.
-
Abstract
- PPC2, together with ABI5 and ABA, is involved in plant acclimation to low CO2 through linking photorespiratory metabolism with primary metabolism.<br />Phosphoenolpyruvate carboxylase (PEPC) plays a pivotal role in the photosynthetic CO2 fixation of C4 plants. However, the functions of PEPCs in C3 plants are less well characterized, particularly in relation to low atmospheric CO2 levels. Of the four genes encoding PEPC in Arabidopsis, PPC2 is considered as the major leaf PEPC gene. Here we show that the ppc2 mutants suffered a growth arrest when transferred to low atmospheric CO2 conditions, together with decreases in the maximum efficiency of PSII (Fv/Fm) and lower levels of leaf abscisic acid (ABA) and carbohydrates. The application of sucrose, malate, or ABA greatly rescued the growth of ppc2 lines under low CO2 conditions. Metabolite profiling analysis revealed that the levels of glycine and serine were increased in ppc2 leaves, while the abundance of photosynthetic metabolites was decreased under these conditions. The transcript levels of encoding enzymes involved in glycine or serine metabolism was decreased in ppc2 in an ABI5-dependent manner. Like the ppc2 mutants, abi5-1 mutants had lower photosynthetic rates and Fv/Fm compared with the wild type under photorespiratory conditions (i.e. low CO2 availability). However, the growth of these mutants was similar to that of the wild type under non-photorespiratory (low O2) conditions. The constitutive expression of ABI5 prevented the growth arrest of ppc2 lines under low CO2 conditions. These findings demonstrate that PPC2 plays an important role in the acclimation of Arabidopsis plants to low CO2 availability by linking photorespiratory metabolism to primary metabolism, and that this is mediated, at least in part, through ABA- and ABI5-dependent processes.
- Subjects :
- 0106 biological sciences
0301 basic medicine
photorespiration
Arabidopsis thaliana
Physiology
Acclimatization
ABI5
PEPC
carbon–nitrogen balance
Plant Science
Photosynthesis
01 natural sciences
Serine
Abscisic acid
03 medical and health sciences
chemistry.chemical_compound
Arabidopsis
Plant Proteins
photosynthesis
biology
AcademicSubjects/SCI01210
Chemistry
organic chemicals
fungi
Wild type
food and beverages
low CO2
Carbon Dioxide
biology.organism_classification
Research Papers
Phosphoenolpyruvate Carboxylase
030104 developmental biology
Biochemistry
Photorespiration
Phosphoenolpyruvate carboxylase
Photosynthesis and Metabolism
010606 plant biology & botany
Subjects
Details
- ISSN :
- 14602431 and 00220957
- Volume :
- 71
- Database :
- OpenAIRE
- Journal :
- Journal of Experimental Botany
- Accession number :
- edsair.doi.dedup.....66000f3bf7b0aa2123d7e54b750cd61b